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Literature summary for 2.7.7.6 extracted from

  • Mani, N.; Dupuy, B.; Sonenshein, A.L.
    Isolation of RNA polymerase from Clostridium difficile and characterization of glutamate dehydrogenase and rRNA gene promoters in vitro and in vivo (2006), J. Bacteriol., 188, 96-102.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis development of an in vitro transcription system that accurately initiates transcription from bona fide promoters of the Peptoclostridium difficile glutamate dehydrogenase gene and rRNA genes rrnC and rrnE operons Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 130000, beta-subunit, plus x * 140000, beta-subunit, SDS-PAGE Clostridioides difficile
140000
-
 x * 130000, beta-subunit, plus x * 140000, beta-subunit, SDS-PAGE Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Clostridioides difficile

Subunits

Subunits Comment Organism
multimer x * 130000, beta-subunit, plus x * 140000, beta'-subunit, SDS-PAGE Clostridioides difficile

General Information

General Information Comment Organism
physiological function RNA polymerase binds to the promoter regions of the gdh, rrnC, and rrnE genes encoding glutamate dehydrogenase and rRNA and activates their transcription Clostridioides difficile