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Literature summary for 2.7.7.6 extracted from
- A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases (2004), Anal. Biochem., 324, 183-190.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | assay for RNA polymerase activity that uses the dye RiboGreen to detect transcripts by fluorescence and is thus free of the expense, short shelf life, and high handling costs of radioisotopes. The method is relatively quick and can be performed entirely in microplate formate, allowing for the processing of dozens to hundreds of samples in parallel | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay for RNA polymerase activity that uses the dye RiboGreen to detect transcripts by fluorescence and is thus free of the expense, short shelf life, and high handling costs of radioisotopes. The method is relatively quick and can be performed entirely in microplate formate, allowing for the processing of dozens to hundreds of samples in parallel | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA-dependent RNA polymerase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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