General Stability | Organism |
---|---|
40% glycerol is absolutely required for stability | Clostridium acetobutylicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rifampicin | 0.1 mg/ml, complete inhibition | Clostridium acetobutylicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ in activation, 10% of the activity observed with Mg2+ | Clostridium acetobutylicum | |
Mg2+ | highest activity is obtained with 20 mM Mg2+ on poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template, with calf thymus DNA as template maximal activity is achieved with 10 mM Mg2+ | Clostridium acetobutylicum | |
Mn2+ | 3 mM Mn2+ results in comparable activity with poly(dA-dT) DNA but reduced activity on calf thymus DNA or Clostridium acetobutylicum DNA and Clostridium acetobutylicum DNA as substrated, compared to Mg2+ activition | Clostridium acetobutylicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | - |
DSM 1731 | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0527 | - |
- |
Clostridium acetobutylicum |
Storage Stability | Organism |
---|---|
-20°C, 60 mM Tris-HCl buffer, pH 7.8, 20 mM NH4Cl, 1 mM EDTA, 10 mM 2-mercaptoethanol, 40% glycerol, stable for several months | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | absolutely dependent on the presence of a double-stranded or single-stranded DNA template | Clostridium acetobutylicum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | with poly(dA-dT) DNA as template | Clostridium acetobutylicum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Clostridium acetobutylicum | diphosphate + RNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | with poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template | Clostridium acetobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
poly(dA-dT) DNA as a template | Clostridium acetobutylicum |