Cloned (Comment) | Organism |
---|---|
expression of glnD in a deficient mutant strain restores the constitutive enzyme, complementation | Corynebacterium glutamicum |
gene glnD, construction of chromosomal deletion mutant | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutant is impaired in its response to nitrogen shortage, mutant shows reduced growth rate in presence of limiting amounts of ammonium or urea, deletion also impairs the transcription of genes amtB and glnK within the same operon | Corynebacterium glutamicum |
additional information | a GlnKY51F protein mutant strain shows no enzyme expression, irrespective of the nitrogen status | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-GlnK] | Corynebacterium glutamicum | uridylylation of GlnK protein is essential for the cell to respond to nitrogen limitation | diphosphate + uridylyl-[protein-GlnK] | - |
? | |
UTP + [protein-PII] | Corynebacterium glutamicum | unlike in enterobacteria, the enzyme is not the primary nitrogen sensor, overexpression leads to derepression of nitrogen control | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | Corynebacterium glutamicum | signal transduction cascade model | diphosphate + uridylyl-[protein-PII] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9X706 | expression is upregulated upon nitrogen limitation | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + [protein-GlnK] | GlnK protein mutant Y51F is no substrate | Corynebacterium glutamicum | diphosphate + uridylyl-[protein-GlnK] | - |
? | |
UTP + [protein-GlnK] | uridylylation of GlnK protein is essential for the cell to respond to nitrogen limitation | Corynebacterium glutamicum | diphosphate + uridylyl-[protein-GlnK] | - |
? | |
UTP + [protein-PII] | - |
Corynebacterium glutamicum | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | unlike in enterobacteria, the enzyme is not the primary nitrogen sensor, overexpression leads to derepression of nitrogen control | Corynebacterium glutamicum | diphosphate + uridylyl-[protein-PII] | - |
? | |
UTP + [protein-PII] | signal transduction cascade model | Corynebacterium glutamicum | diphosphate + uridylyl-[protein-PII] | - |
? |