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Literature summary for 2.7.7.59 extracted from

  • Schlüter, A.; Nohlen, M.; Kramer, M.; Defez, R.; Priefer, U.B.
    The Rhizobium leguminosarum bv. viciae glnD gene, encoding a uridylyltransferase/uridylyl-removing enzyme, is expressed in the root nodule but is not essential for nitrogen fixation (2000), Microbiology, 146, 2987-2996.
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Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, recombination of mutant gene into genomic glnD region via double crossover, mutnat VF39-BB3 and VF39-BB1 Rhizobium leguminosarum

Protein Variants

Protein Variants Comment Organism
additional information mutant construction by transposon Tn5 insertion and by subcloning + double crossover for recombination, phenotype characterization. Mutants are unable to utilize nitrate, essential function of glnD since most mutations close to the 5'-end are lethal Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + [protein-PII] Rhizobium leguminosarum enzyme is not essential for nitrogen fixation of the host plant diphosphate + uridylyl-[protein-PII]
-
 ?
UTP + [protein-PII] Rhizobium leguminosarum the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system diphosphate + uridylyl-[protein-PII]
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum Q9RAE4 from root nodules of Vicia hirsuta
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Rhizobium leguminosarum Q9RAE4 bv. viciae, constitutive gene glnD
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Source Tissue

Source Tissue Comment Organism Textmining
root nodule enzyme expression Rhizobium leguminosarum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + [protein-PII]
-
 Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
-
 ?
UTP + [protein-PII] enzyme is not essential for nitrogen fixation of the host plant Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
-
 ?
UTP + [protein-PII] the enzyme acts as the primary nitrogen sensor in the nitrogen regulation system Rhizobium leguminosarum diphosphate + uridylyl-[protein-PII]
-
 ?