Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method. The 1.9 A crystal structures of the apo and the phosphate-bound forms of RNase PH from reveal a monomeric RNase PH with an alpha/beta-fold tightly associated into a hexameric ring structure in the form of a trimer of dimers | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
R127A | mutation has no influence on the structure | Pseudomonas aeruginosa |
R69S | mutation has no influence on the structure | Pseudomonas aeruginosa |
R69S/R77S | mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure | Pseudomonas aeruginosa |
R74S | mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure | Pseudomonas aeruginosa |
R74S/R77S | mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure | Pseudomonas aeruginosa |
R77S | mutation has no influence on the structure | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAn+1 + phosphate | Pseudomonas aeruginosa | catalyzes the removal of nucleotides at the 3' end of the tRNA precursor, leading to the release of nucleoside diphosphate, and generates the CCA end during the maturation process | tRNAn + a nucleoside diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAn+1 + phosphate | catalyzes the removal of nucleotides at the 3' end of the tRNA precursor, leading to the release of nucleoside diphosphate, and generates the CCA end during the maturation process | Pseudomonas aeruginosa | tRNAn + a nucleoside diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 1.9 A crystal structures of the apo and the phosphate-bound forms of RNase PH from reveal a monomeric RNase PH with an alpha/beta-fold tightly associated into a hexameric ring structure in the form of a trimer of dimers | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
Tm-value for mutant enzymes R69S/R77S, R74S/R77S and R74S | Pseudomonas aeruginosa |
52 | - |
Tm-value for wild-type enzyme and mutant enzyme R127A | Pseudomonas aeruginosa |