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Literature summary for 2.7.7.56 extracted from
- Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli (1992), J. Biol. Chem., 267, 17147-17152.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rph, overexpression in strain NF1815 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
sucrose density gradient centrifugation | Escherichia coli |
| 200000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNAn+1 + phosphate | Escherichia coli | brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate | tRNA + a nucleoside diphosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene rph, orfE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from overproducing strain NF1815 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | - |
formation of CDP from tRNA-C-C-A-Cn | Escherichia coli |
| 1.6 | - |
phosphorolytic cleavage of poly(A) | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) + phosphate | - |
Escherichia coli | ? | - |
r | |
| tRNAn+1 + phosphate | tRNA-C-C-A-Cn | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
| tRNAn+1 + phosphate | brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in E. coli by phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | native protein is composed of 2 or more subunits | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
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