BRENDA - Enzyme Database
show all sequences of 2.7.7.53

Structural insights into the novel diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

Mori, S.; Shibayama, K.; Wachino, J.; Arakawa, Y.; J. Mol. Biol. 410, 93-104 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
crystal structure at 1.89 A resolution by using single-wavelength anomalous dispersion. Asn139, Gly146, and Ser147 are important active-site residues, Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. The tetrameric structure and the presence of Trp160 might be essential for the formation of the Ap4A binding site
Mycobacterium tuberculosis
Engineering
Amino acid exchange
Commentary
Organism
G146Q
about 20% of wild-type activity
Mycobacterium tuberculosis
G146S
about 30% of wild-type activity
Mycobacterium tuberculosis
N139A
complete loss of activity
Mycobacterium tuberculosis
N139Q
complete loss of activity
Mycobacterium tuberculosis
N139Q/S147T
complete loss of activity
Mycobacterium tuberculosis
S147A
about 8% of wild-type activity
Mycobacterium tuberculosis
S147T
about 15% of wild-type activity
Mycobacterium tuberculosis
W160A
complete loss of activity
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
0.08
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C; mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
0.1
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
0.11
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WMK9
-
-
Mycobacterium tuberculosis H37Rv
P9WMK9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
722980
Mycobacterium tuberculosis
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
722980
Mycobacterium tuberculosis H37Rv
ADP + ATP
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.48
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
1.38
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C
Mycobacterium tuberculosis
1.49
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
1.99
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
8.48
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure at 1.89 A resolution by using single-wavelength anomalous dispersion. Asn139, Gly146, and Ser147 are important active-site residues, Asn139 has a critical role in catalysis. The position of Gly146 might influence the phosphorylase activity. The tetrameric structure and the presence of Trp160 might be essential for the formation of the Ap4A binding site
Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G146Q
about 20% of wild-type activity
Mycobacterium tuberculosis
G146S
about 30% of wild-type activity
Mycobacterium tuberculosis
N139A
complete loss of activity
Mycobacterium tuberculosis
N139Q
complete loss of activity
Mycobacterium tuberculosis
N139Q/S147T
complete loss of activity
Mycobacterium tuberculosis
S147A
about 8% of wild-type activity
Mycobacterium tuberculosis
S147T
about 15% of wild-type activity
Mycobacterium tuberculosis
W160A
complete loss of activity
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
0.08
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C; mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
0.1
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
0.11
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
722980
Mycobacterium tuberculosis
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
722980
Mycobacterium tuberculosis H37Rv
ADP + ATP
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.48
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
1.38
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C
Mycobacterium tuberculosis
1.49
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
1.99
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
8.48
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
13.6
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
17.25
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C
Mycobacterium tuberculosis
24.88
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
84.8
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147A, pH 7.6, 37C
Mycobacterium tuberculosis
13.6
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant S147T, pH 7.6, 37C
Mycobacterium tuberculosis
17.25
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146Q, pH 7.6, 37C
Mycobacterium tuberculosis
24.88
-
P1,P4-bis(5'-adenosyl) tetraphosphate
mutant G146S, pH 7.6, 37C
Mycobacterium tuberculosis
84.8
-
P1,P4-bis(5'-adenosyl) tetraphosphate
wild-type, pH 7.6, 37C
Mycobacterium tuberculosis
Other publictions for EC 2.7.7.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737871
Mori
Roles of Ala-149 in the cataly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biosci. Biotechnol. Biochem.
79
236-238
2015
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739577
Honda
Purification and functional ch ...
Mycobacterium avium 104, Mycobacterium avium, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Protein Expr. Purif.
112
37-42
2015
-
-
2
-
-
-
-
-
-
8
2
-
-
7
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722980
Mori
Structural insights into the n ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
410
93-104
2011
-
-
1
1
8
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
713455
Mori
Purification and molecular cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
69
99-105
2010
-
-
1
-
-
-
-
2
-
4
2
-
-
6
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
2
-
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
643515
McLennan
Anabaena flos-aquae and other ...
Dolichospermum flos-aquae, Synechococcus sp., Trichormus variabilis
Biochem. J.
320
795-800
1996
-
-
-
-
-
-
-
1
-
5
3
-
-
10
-
-
1
-
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
3
-
-
-
-
1
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Acanthamoeba castellanii
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643514
Booth
An alleged yeast polyphosphate ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
19377-19382
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209909
McLennan
The green alga Scenedesmus obl ...
Chlorella vulgaris, Scenedesmus basiliensis, Scenedesmus quadricauda, Tetradesmus obliquus
Biochem. J.
300
183-189
1994
-
-
-
-
-
-
1
7
-
7
2
-
-
9
-
-
1
-
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
7
2
-
-
-
-
1
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
643509
Avila
Immunoaffinity chromatography ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CGY 339
Biotechnol. Appl. Biochem.
12
276-283
1990
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
643513
Guranowski
Specific phosphorylase from Eu ...
Acanthamoeba castellanii, Euglena gracilis
Int. J. Biochem.
20
449-455
1988
-
-
-
-
-
-
-
3
-
2
1
-
-
2
-
-
1
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Saccharomyces cerevisiae
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643508
Brevet
Yeast diadenosine 5,5-P1,P4-te ...
Saccharomyces cerevisiae
Biochemistry
26
4763-4768
1987
-
-
-
-
-
-
1
2
-
6
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
6
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643510
Guranowski
Diadenosine 5,5-P1, P4-tetraph ...
Saccharomyces cerevisiae
J. Biol. Chem.
261
5943-5946
1986
-
-
-
-
-
2
8
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
4
-
2
-
-
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-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
643507
Guranowski
Phosphorolytic cleavage of dia ...
Saccharomyces cerevisiae
J. Biol. Chem.
260
3542-3547
1985
-
-
-
-
-
1
4
3
-
8
1
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
8
1
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-