Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C | Mycobacterium tuberculosis | |
0.94 | - |
phosphate | in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Rv2613c requires bivalent metal ions such as Ca2+ to exert its Ap4A phosphorylase activity (64% activity in the presence of 2 mM Ca2+) | Mycobacterium tuberculosis | |
Co2+ | Rv2613c requires bivalent metal ions such as Co2+ to exert its Ap4A phosphorylase activity (68% activity in the presence of 2 mM Co2+) | Mycobacterium tuberculosis | |
Mg2+ | Rv2613c requires bivalent metal ions such as Mg2+ to exert its Ap4A phosphorylase activity (34% activity in the presence of 2 mM Mg2+) | Mycobacterium tuberculosis | |
Mn2+ | Rv2613c requires bivalent metal ions such as Mn2+ to exert its Ap4A phosphorylase activity (100% activity in the presence of 2 mM Mn2+) | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24683 | - |
4 * 24683, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
100000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMK9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WMK9 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Sephacryl S-200 gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.01 | - |
crude enzyme, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C | Mycobacterium tuberculosis |
10.84 | - |
after 3.6fold purification, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows no hydrolysis of AMP-NH2, and almost no activity with adenosine tetraphosphate, ATP, ADP-ribose, ADP-glucose, guanosine tetraphosphate, guanosine 5'-triphosphate, GDP-glucose, GDP-mannose, adenylyl imidodiphosphate, and guanylyl imidodiphosphate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows no hydrolysis of AMP-NH2, and almost no activity with adenosine tetraphosphate, ATP, ADP-ribose, ADP-glucose, guanosine tetraphosphate, guanosine 5'-triphosphate, GDP-glucose, GDP-mannose, adenylyl imidodiphosphate, and guanylyl imidodiphosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
P1,P3-bis(5'-adenosyl) triphosphate + phosphate | 19% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | ADP | - |
? | |
P1,P3-bis(5'-adenosyl) triphosphate + phosphate | 19% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis H37Rv | ADP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | 100% activity | Mycobacterium tuberculosis | ATP + ADP | - |
ir | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | 100% activity | Mycobacterium tuberculosis H37Rv | ATP + ADP | - |
ir | |
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate | 107% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | GTP + GDP | - |
? | |
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate | 107% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis H37Rv | GTP + GDP | - |
? | |
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate | 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | adenosine tetraphosphate + ADP | - |
? | |
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate | 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis H37Rv | adenosine tetraphosphate + ADP | - |
? | |
P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate | 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | guanosine tetraphosphate + GDP | - |
? | |
P1,P6-bis(5'-adenosyl) hexaphosphate + phosphate | 25% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | adenosine pentaphosphate + ADP | - |
? | |
P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate | 100% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | ATP + GDP | - |
? | |
P1-(5'-adenosyl)P5-(5'-guanosyl) pentaphosphate + phosphate | 76% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate | Mycobacterium tuberculosis | adenosine tetraphosphate + GDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 24683, MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase | - |
Mycobacterium tuberculosis |
Rv2613c | the enzyme has Ap4A phosphorylase activity rather than Ap4A hydrolase activity | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the activity completely disappears after treatment at 65°C for 10 min | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 10 | - |
Mycobacterium tuberculosis |