BRENDA - Enzyme Database
show all sequences of 2.7.7.53

Anabaena flos-aquae and other cyanobacteria possess diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) phosphorylase activity

McLennan, A.G.; Mayers, E.; Adams, D.G.; Biochem. J. 320, 795-800 (1996)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 9.5
Dolichospermum flos-aquae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Dolichospermum flos-aquae
Co2+
activates
Dolichospermum flos-aquae
Mg2+
required, most effective divalent cation
Dolichospermum flos-aquae
Mn2+
activates
Dolichospermum flos-aquae
Zn2+
slight activation
Dolichospermum flos-aquae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Dolichospermum flos-aquae
32000
-
gel filtration
Trichormus variabilis
39000
-
gel filtration
Synechococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dolichospermum flos-aquae
-
-
-
Synechococcus sp.
-
-
-
Trichormus variabilis
-
-
-
Purification (Commentary)
Commentary
Organism
partial, 206fold
Dolichospermum flos-aquae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.037
-
partially purified enzyme
Dolichospermum flos-aquae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
P1,P6-bis(5'-adenosyl) hexaphosphate is a very poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
-
additional information
no activity with sulfate
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP
-
643515
Dolichospermum flos-aquae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
182% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + ATP
-
643515
Dolichospermum flos-aquae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Synechococcus sp.
ADP + ATP
-
643515
Synechococcus sp.
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Trichormus variabilis
ADP + ATP
-
643515
Trichormus variabilis
?
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
225% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + p4A
-
643515
Dolichospermum flos-aquae
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Dolichospermum flos-aquae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 9.5
Dolichospermum flos-aquae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Dolichospermum flos-aquae
Co2+
activates
Dolichospermum flos-aquae
Mg2+
required, most effective divalent cation
Dolichospermum flos-aquae
Mn2+
activates
Dolichospermum flos-aquae
Zn2+
slight activation
Dolichospermum flos-aquae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Dolichospermum flos-aquae
32000
-
gel filtration
Trichormus variabilis
39000
-
gel filtration
Synechococcus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 206fold
Dolichospermum flos-aquae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.037
-
partially purified enzyme
Dolichospermum flos-aquae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
P1,P6-bis(5'-adenosyl) hexaphosphate is a very poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
-
additional information
no activity with sulfate
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP
-
643515
Dolichospermum flos-aquae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
182% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
poor substrate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + ATP
-
643515
Dolichospermum flos-aquae
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Synechococcus sp.
ADP + ATP
-
643515
Synechococcus sp.
?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643515
Trichormus variabilis
ADP + ATP
-
643515
Trichormus variabilis
?
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
no activity
643515
Dolichospermum flos-aquae
?
-
-
-
-
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
225% activity compared to phosphate
643515
Dolichospermum flos-aquae
?
-
-
-
r
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
-
643515
Dolichospermum flos-aquae
ADP + p4A
-
643515
Dolichospermum flos-aquae
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Dolichospermum flos-aquae
Other publictions for EC 2.7.7.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737871
Mori
Roles of Ala-149 in the cataly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biosci. Biotechnol. Biochem.
79
236-238
2015
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739577
Honda
Purification and functional ch ...
Mycobacterium avium 104, Mycobacterium avium, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Protein Expr. Purif.
112
37-42
2015
-
-
2
-
-
-
-
-
-
8
2
-
-
7
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722980
Mori
Structural insights into the n ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
410
93-104
2011
-
-
1
1
8
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
713455
Mori
Purification and molecular cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
69
99-105
2010
-
-
1
-
-
-
-
2
-
4
2
-
-
6
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
2
-
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
643515
McLennan
Anabaena flos-aquae and other ...
Dolichospermum flos-aquae, Synechococcus sp., Trichormus variabilis
Biochem. J.
320
795-800
1996
-
-
-
-
-
-
-
1
-
5
3
-
-
10
-
-
1
-
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
3
-
-
-
-
1
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Acanthamoeba castellanii
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643514
Booth
An alleged yeast polyphosphate ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
19377-19382
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209909
McLennan
The green alga Scenedesmus obl ...
Chlorella vulgaris, Scenedesmus basiliensis, Scenedesmus quadricauda, Tetradesmus obliquus
Biochem. J.
300
183-189
1994
-
-
-
-
-
-
1
7
-
7
2
-
-
9
-
-
1
-
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
7
2
-
-
-
-
1
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
643509
Avila
Immunoaffinity chromatography ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CGY 339
Biotechnol. Appl. Biochem.
12
276-283
1990
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
643513
Guranowski
Specific phosphorylase from Eu ...
Acanthamoeba castellanii, Euglena gracilis
Int. J. Biochem.
20
449-455
1988
-
-
-
-
-
-
-
3
-
2
1
-
-
2
-
-
1
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Saccharomyces cerevisiae
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643508
Brevet
Yeast diadenosine 5,5-P1,P4-te ...
Saccharomyces cerevisiae
Biochemistry
26
4763-4768
1987
-
-
-
-
-
-
1
2
-
6
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
6
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643510
Guranowski
Diadenosine 5,5-P1, P4-tetraph ...
Saccharomyces cerevisiae
J. Biol. Chem.
261
5943-5946
1986
-
-
-
-
-
2
8
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
4
-
2
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
643507
Guranowski
Phosphorolytic cleavage of dia ...
Saccharomyces cerevisiae
J. Biol. Chem.
260
3542-3547
1985
-
-
-
-
-
1
4
3
-
8
1
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
8
1
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-