BRENDA - Enzyme Database
show all sequences of 2.7.7.53

Specific phosphorylase from Euglena gracilis splits diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) and diadenosine 5',5'''-P1,P3-tetraphosphate (Ap3A)

Guranowski, A.; Starzynska, E.; Wasternack, C.; Int. J. Biochem. 20, 449-455 (1988)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
P1,P3-bis(5'-adenosyl) triphosphate
-
Euglena gracilis
0.027
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Euglena gracilis
0.5
-
phosphate
-
Euglena gracilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
divalent metal cations
requirement
Euglena gracilis
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Euglena gracilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
-
Euglena gracilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acanthamoeba castellanii
-
-
-
Euglena gracilis
-
-
-
Purification (Commentary)
Commentary
Organism
625fold
Euglena gracilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NDP + arsenate
-
643513
Euglena gracilis
NMP + phosphate + ?
-
643513
Euglena gracilis
?
NDP + phosphate
-
643513
Euglena gracilis
phosphate + NDP
-
643513
Euglena gracilis
r
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Euglena gracilis
ADP
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Acanthamoeba castellanii
ADP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Euglena gracilis
ADP + ATP
-
643513
Euglena gracilis
-
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Acanthamoeba castellanii
ADP + ATP
-
643513
Acanthamoeba castellanii
?
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate
i.e. ApppCH2pA, methylene group substituting the oxygen
643513
Euglena gracilis
?
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643513
Euglena gracilis
GDP + GTP
-
-
-
?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643513
Euglena gracilis
ADP + p4A
-
-
-
?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643513
Euglena gracilis
ADP + p4A
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Euglena gracilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
P1,P3-bis(5'-adenosyl) triphosphate
-
Euglena gracilis
0.027
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Euglena gracilis
0.5
-
phosphate
-
Euglena gracilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
divalent metal cations
requirement
Euglena gracilis
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Euglena gracilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
-
Euglena gracilis
Purification (Commentary) (protein specific)
Commentary
Organism
625fold
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NDP + arsenate
-
643513
Euglena gracilis
NMP + phosphate + ?
-
643513
Euglena gracilis
?
NDP + phosphate
-
643513
Euglena gracilis
phosphate + NDP
-
643513
Euglena gracilis
r
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Euglena gracilis
ADP
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Acanthamoeba castellanii
ADP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Euglena gracilis
ADP + ATP
-
643513
Euglena gracilis
-
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Acanthamoeba castellanii
ADP + ATP
-
643513
Acanthamoeba castellanii
?
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
643513
Euglena gracilis
?
-
-
-
r
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate
i.e. ApppCH2pA, methylene group substituting the oxygen
643513
Euglena gracilis
?
-
-
-
?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643513
Euglena gracilis
GDP + GTP
-
-
-
?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643513
Euglena gracilis
ADP + p4A
-
-
-
?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643513
Euglena gracilis
ADP + p4A
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Euglena gracilis
Other publictions for EC 2.7.7.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737871
Mori
Roles of Ala-149 in the cataly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biosci. Biotechnol. Biochem.
79
236-238
2015
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739577
Honda
Purification and functional ch ...
Mycobacterium avium 104, Mycobacterium avium, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Protein Expr. Purif.
112
37-42
2015
-
-
2
-
-
-
-
-
-
8
2
-
-
7
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
-
-
-
-
-
-
28
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722980
Mori
Structural insights into the n ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
410
93-104
2011
-
-
1
1
8
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
713455
Mori
Purification and molecular cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
69
99-105
2010
-
-
1
-
-
-
-
2
-
4
2
-
-
6
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
2
-
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
643515
McLennan
Anabaena flos-aquae and other ...
Dolichospermum flos-aquae, Synechococcus sp., Trichormus variabilis
Biochem. J.
320
795-800
1996
-
-
-
-
-
-
-
1
-
5
3
-
-
10
-
-
1
-
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
3
-
-
-
-
1
-
-
1
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Acanthamoeba castellanii
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
4
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643514
Booth
An alleged yeast polyphosphate ...
Saccharomyces cerevisiae
J. Biol. Chem.
270
19377-19382
1995
-
-
-
-
-
-
3
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209909
McLennan
The green alga Scenedesmus obl ...
Chlorella vulgaris, Scenedesmus basiliensis, Scenedesmus quadricauda, Tetradesmus obliquus
Biochem. J.
300
183-189
1994
-
-
-
-
-
-
1
7
-
7
2
-
-
9
-
-
1
-
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
7
2
-
-
-
-
1
-
-
5
1
25
1
5
-
-
-
6
1
-
-
-
-
-
-
-
-
643509
Avila
Immunoaffinity chromatography ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae CGY 339
Biotechnol. Appl. Biochem.
12
276-283
1990
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
643513
Guranowski
Specific phosphorylase from Eu ...
Acanthamoeba castellanii, Euglena gracilis
Int. J. Biochem.
20
449-455
1988
-
-
-
-
-
-
-
3
-
2
1
-
-
2
-
-
1
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
-
1
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Saccharomyces cerevisiae
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643508
Brevet
Yeast diadenosine 5,5-P1,P4-te ...
Saccharomyces cerevisiae
Biochemistry
26
4763-4768
1987
-
-
-
-
-
-
1
2
-
6
-
1
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
6
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643510
Guranowski
Diadenosine 5,5-P1, P4-tetraph ...
Saccharomyces cerevisiae
J. Biol. Chem.
261
5943-5946
1986
-
-
-
-
-
2
8
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
4
-
2
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
1
4
-
-
-
-
-
-
-
-
-
643507
Guranowski
Phosphorolytic cleavage of dia ...
Saccharomyces cerevisiae
J. Biol. Chem.
260
3542-3547
1985
-
-
-
-
-
1
4
3
-
8
1
-
-
1
-
-
1
-
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
8
1
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-