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Literature summary for 2.7.7.53 extracted from

  • Guranowski, A.; Starzynska, E.; Wasternack, C.
    Specific phosphorylase from Euglena gracilis splits diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) and diadenosine 5',5'''-P1,P3-tetraphosphate (Ap3A) (1988), Int. J. Biochem., 20, 449-455.
    View publication on PubMed
Search for more literature for 2.7.7.53

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 P1,P3-bis(5'-adenosyl) triphosphate
-
 Euglena gracilis
0.027
-
 P1,P4-bis(5'-adenosyl) tetraphosphate
-
 Euglena gracilis
0.5
-
 phosphate
-
 Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
divalent metal cations requirement Euglena gracilis
additional information no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
-
 Euglena gracilis

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii
-
-
-
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
625fold Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NDP + arsenate
-
 Euglena gracilis NMP + phosphate + ?
-
 ?
NDP + phosphate
-
 Euglena gracilis phosphate + NDP
-
 r
P1,P3-bis(5'-adenosyl) triphosphate + phosphate i.e. AP3A Euglena gracilis ADP
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate i.e. AP3A Acanthamoeba castellanii ADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
 Euglena gracilis ?
-
 r
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
 Euglena gracilis ?
-
 r
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate i.e. Ap4A or AppppA, substrate specificity Euglena gracilis ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate i.e. Ap4A or AppppA, substrate specificity Acanthamoeba castellanii ADP + ATP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
 Euglena gracilis ?
-
 r
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate i.e. ApppCH2pA, methylene group substituting the oxygen Euglena gracilis ?
-
 ?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate i.e. GP4G Euglena gracilis GDP + GTP
-
 ?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate arsenate can replace phosphate Euglena gracilis ADP + p4A
-
 ?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate i.e. AP5A Euglena gracilis ADP + p4A
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Euglena gracilis