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Literature summary for 2.7.7.53 extracted from

  • Guranowski, A.; Blanquet, S.
    Phosphorolytic cleavage of diadenosine 5,5-P1,P4-tetraphosphate. Properties of homogeneous diadenosine 5,5-P1,P4-tetraphosphate alpha, beta-phosphorylase from Saccharomyces cerevisiae (1985), J. Biol. Chem., 260, 3542-3547.
    View publication on PubMed
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General Stability

General Stability Organism
glycerol, 10%, stabilizes Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
dithioerythritol
-
 Saccharomyces cerevisiae
iodoacetate
-
 Saccharomyces cerevisiae
N-ethylmaleimide
-
 Saccharomyces cerevisiae
p-hydroxymercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 8.2, 37°C Saccharomyces cerevisiae
1
-
 phosphate pH 8.2, 37°C Saccharomyces cerevisiae
3
-
 arsenate pH 8.2, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ less effective than Mg2+ Saccharomyces cerevisiae
Ca2+ requirement, less effective than Mn2+ Saccharomyces cerevisiae
Cd2+ slight activation Saccharomyces cerevisiae
Co2+ activation, less effective than Mn2+ or Ca2+ or Mg2+ Saccharomyces cerevisiae
divalent metal cations requirement Saccharomyces cerevisiae
Mg2+ required Saccharomyces cerevisiae
Mn2+ required Saccharomyces cerevisiae
additional information no activation by Ni2+ Saccharomyces cerevisiae
Zn2+ no effect Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
55
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are 3'-adenosyltetraphosphate, poly(A), 3',5'-cAMP, NADP+, P1-bis(5'-adenosyl) monophosphate Ap2A, thymidine 5'-monophosphate-p-nitrophenylester Saccharomyces cerevisiae ?
-
 ?
additional information no activity with sulfate Saccharomyces cerevisiae ?
-
 ?
additional information no substrates are ADP, ATP, NAD+ Saccharomyces cerevisiae ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate no activity with Saccharomyces cerevisiae ADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate arsenolysis, at about 80% the rate of phosphorolysis Saccharomyces cerevisiae ?
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Saccharomyces cerevisiae ADP + ATP
-
 ?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate arsenate can replace phosphate Saccharomyces cerevisiae GDP + GTP
-
 ?
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate i.e. GP4G Saccharomyces cerevisiae GDP + GTP
-
 ?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate arsenate can replace phosphate Saccharomyces cerevisiae ADP + p4A
-
 ?
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate i.e. AP5A Saccharomyces cerevisiae ADP + p4A
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 about half-maximal activity at pH 6.5 and pH 9.0, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis Saccharomyces cerevisiae