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Literature summary for 2.7.7.53 extracted from

  • Guranowski, A.; Starzynska, E.; Gzik, L.; Langston, S.P.; Brown, P.; Blackburn, G.M.
    Methylene and halomethylene analogues of diadenosine 5'5'''-P1,P3-triphosphate (ApppA) as substrates or inhibitors of ApppA-degrading enzymes (1995), Nucleosides Nucleotides, 14, 731-734.
No PubMed abstract available
Search for more literature for 2.7.7.53

Inhibitors

Inhibitors Comment Organism Structure
P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2 i.e. ApCCl2ppA Acanthamoeba castellanii
P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2 i.e. ApCHClppA Acanthamoeba castellanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 pH 8.0, 30°C Acanthamoeba castellanii
0.004
-
 P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 pH 8.0, 30°C Acanthamoeba castellanii
0.009
-
 P1,P3-bis(5'-adenosyl) triphosphate pH 8.0, 30°C Acanthamoeba castellanii
0.012
-
 P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 pH 8.0, 30°C Acanthamoeba castellanii

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Acanthamoeba castellanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme splits the anhydride bond between the alpha and beta phosphates by phosphorolysis Acanthamoeba castellanii ?
-
 ?
additional information no activity with P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1and P2 and between P3 and P4, i.e. ApCH2pCH2pA Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate preferred substrate Acanthamoeba castellanii ADP
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate + phosphate i.e. AP3A Acanthamoeba castellanii ADP
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate low activity Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CF2-bridge between P1 and P2 + phosphate ApCF2ppA, difluoromethylene group substituting the oxygen Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate poor substrate Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CH2-bridge between P1 and P2 + phosphate i.e. ApCH2ppA, methylene group substituting the oxygen Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate low activity Acanthamoeba castellanii ?
-
 ?
P1,P3-bis(5'-adenosyl) triphosphate with CHF-bridge between P1 and P2 + phosphate i.e. ApCHFppA, fluoromethylene group substituting the oxygen Acanthamoeba castellanii ?
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
 Acanthamoeba castellanii ADP + ATP
-
 ?

Synonyms

Synonyms Comment Organism
Ap3A/Ap4A phosphorylase
-
 Acanthamoeba castellanii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
 P1,P3-bis(5'-adenosyl) triphosphate with CHCl-bridge between P1 and P2 pH 8.0, 30°C Acanthamoeba castellanii
0.014
-
 P1,P3-bis(5'-adenosyl) triphosphate with CCl2-bridge between P1 and P2 pH 8.0, 30°C Acanthamoeba castellanii