Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by F- or adenosine 5'-tetraphosphate | Tetradesmus obliquus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
0.07 | - |
vanadate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
0.13 | - |
arsenate | P1,P4-bis(5'-adenosyl) tetraphosphate-arsenolysis, pH 7.4, 25°C | Tetradesmus obliquus | |
0.16 | - |
phosphate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
0.45 | - |
molybdate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
0.52 | - |
chromate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus | |
1.75 | - |
tungstate | P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25°C | Tetradesmus obliquus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | less effective than Mg2+ | Tetradesmus obliquus | |
Ca2+ | as good as Mn2+ | Tetradesmus obliquus | |
Ca2+ | less effective than Co2+ | Tetradesmus obliquus | |
Cd2+ | slight activation | Tetradesmus obliquus | |
Co2+ | activation, less effective than Mn2+ or Ca2+ or Mg2+ | Tetradesmus obliquus | |
divalent metal cations | requirement | Tetradesmus obliquus | |
Mg2+ | - |
Tetradesmus obliquus | |
Mn2+ | 10 mM | Tetradesmus obliquus | |
Mn2+ | less effective than Mg2+ or Co2+ | Tetradesmus obliquus | |
Zn2+ | slight activation | Tetradesmus obliquus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000, SDS-PAGE | Tetradesmus obliquus |
46000 | 48000 | gel filtration | Tetradesmus obliquus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella vulgaris | - |
green alga | - |
Scenedesmus basiliensis | - |
green alga | - |
Scenedesmus quadricauda | - |
green alga | - |
Tetradesmus obliquus | - |
green alga | - |
Purification (Comment) | Organism |
---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold | Tetradesmus obliquus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00014 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus quadricauda |
0.00065 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
0.00096 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Chlorella vulgaris |
0.0012 | - |
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus basiliensis |
1.96 | - |
purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
Storage Stability | Organism |
---|---|
-20°C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable | Tetradesmus obliquus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + arsenate | - |
Tetradesmus obliquus | AMP + phosphate + ? | - |
? | |
ADP + ATP | - |
Tetradesmus obliquus | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
ADP + ATP | - |
Scenedesmus basiliensis | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
ADP + ATP | - |
Chlorella vulgaris | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
ADP + ATP | - |
Scenedesmus quadricauda | P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
r | |
ADP + phosphate | ADP/phosphate exchange reaction | Tetradesmus obliquus | phosphate + ADP | - |
r | |
additional information | no substrates are AMP, p-nitrophenylthymidine 5'-triphosphate, bis-p-nitrophenyl phosphate | Tetradesmus obliquus | ? | - |
? | |
additional information | no activity with sulfate | Tetradesmus obliquus | ? | - |
? | |
additional information | no activity with sulfate | Scenedesmus basiliensis | ? | - |
? | |
additional information | no activity with sulfate | Chlorella vulgaris | ? | - |
? | |
additional information | no activity with sulfate | Scenedesmus quadricauda | ? | - |
? | |
additional information | no substrates are ADP, ATP, NAD+ | Tetradesmus obliquus | ? | - |
? | |
P1,P3-bis(5'-adenosyl) triphosphate + phosphate | no activity with | Tetradesmus obliquus | ADP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Tetradesmus obliquus | ? | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Scenedesmus basiliensis | ? | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Chlorella vulgaris | ? | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate | - |
Scenedesmus quadricauda | ? | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate | - |
Tetradesmus obliquus | ? | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate | - |
Tetradesmus obliquus | ? | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Tetradesmus obliquus | ADP + ATP | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Scenedesmus basiliensis | ADP + ATP | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Chlorella vulgaris | ADP + ATP | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate | - |
Scenedesmus quadricauda | ADP + ATP | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate | - |
Tetradesmus obliquus | ? | - |
r | |
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate | - |
Tetradesmus obliquus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, SDS-PAGE | Tetradesmus obliquus |
Synonyms | Comment | Organism |
---|---|---|
Ap4A phosphorylase | - |
Tetradesmus obliquus |
Ap4A phosphorylase | - |
Scenedesmus basiliensis |
Ap4A phosphorylase | - |
Chlorella vulgaris |
Ap4A phosphorylase | - |
Scenedesmus quadricauda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Tetradesmus obliquus |
25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus basiliensis |
25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Chlorella vulgaris |
25 | - |
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis | Scenedesmus quadricauda |
30 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at | Tetradesmus obliquus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at | Tetradesmus obliquus |
7.5 | 9.5 | - |
Tetradesmus obliquus |
8 | - |
assay at | Tetradesmus obliquus |
8 | - |
assay at | Scenedesmus basiliensis |
8 | - |
assay at | Chlorella vulgaris |
8 | - |
assay at | Scenedesmus quadricauda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.8 | about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8 | Tetradesmus obliquus |