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Literature summary for 2.7.7.52 extracted from

  • Chung, C.Z.; Jo, D.H.; Heinemann, I.U.
    Nucleotide specificity of the human terminal nucleotidyltransferase Gld2 (TUT2) (2016), RNA, 22, 1239-1249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000228
-
 ATP pH 8.0, 37°C Homo sapiens
0.055
-
 CTP pH 8.0, 37°C Homo sapiens
0.169
-
 UTP pH 8.0, 37°C Homo sapiens
0.23
-
 GTP pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (mRNA)n i.e. total human mRNA Homo sapiens diphosphate + (mRNA)n+1
-
 ?
ATP + 5'(ppp)-UGAGGUAGUAGGUUGUAUAGUU-3' i.e. triphosphorylated human let-7a-5p-3p Homo sapiens diphosphate + 5'(ppp)-UGAGGUAGUAGGUUGUAUAGUUA-3'
-
 ?
ATP + 5'-UGAGGUAGUAGGUUGUAUAGUU-3' i.e. unphosphorylated human let-7a-0P. Gld2 displays an 83fold preference of ATP over UTP Homo sapiens diphosphate + 5'-UGAGGUAGUAGGUUGUAUAGUUA-3'
-
 ?
CTP + 5'(pp)-UGAGGUAGUAGGUUGUAUAGUU-3' i.e. diphosphorylated human let-7a-5p-2p Homo sapiens diphosphate + 5'(pp)-UGAGGUAGUAGGUUGUAUAGUUC-3'
-
 ?
GTP + miR-122
-
 Homo sapiens diphosphate + ?
-
 ?
additional information human Gld2 is a bona fide adenylyltransferase with only weak activity toward other nucleotides. Gld2 is a promiscuous enzyme, with activity toward miRNA, pre-miRNA, and polyadenylated RNA substrates. Gld2 shows a clear preference for ATP in the presence of all four NTPs. Apo-Gld2 activity is restricted to adding single nucleotides and processivity likely relies on additional RNA-binding proteins Homo sapiens ?
-
 ?
UTP + 5'(p)-UGAGGUAGUAGGUUGUAUAGUU-3' i.e. monophosphorylated human let-7a-5p. Gld2 displays an 83fold preference of ATP over UTP Homo sapiens diphosphate + 5'(p)-UGAGGUAGUAGGUUGUAUAGUUU-3'
-
 ?

Synonyms

Synonyms Comment Organism
GLD2
-
 Homo sapiens
TUT2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000293
-
 ATP pH 8.0, 37°C Homo sapiens
0.000248
-
 UTP pH 8.0, 37°C Homo sapiens
0.000403
-
 GTP pH 8.0, 37°C Homo sapiens
0.000914
-
 CTP pH 8.0, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0015
-
 UTP pH 8.0, 37°C Homo sapiens
0.0018
-
 GTP pH 8.0, 37°C Homo sapiens
0.0166
-
 CTP pH 8.0, 37°C Homo sapiens
0.128
-
 ATP pH 8.0, 37°C Homo sapiens