Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.52 extracted from

  • Aphasizhev, R.; Sbicego, S.; Peris, M.; Jang, S.H.; Aphasizheva, I.; Simpson, A.M.; Rivlin, A.; Simpson, L.
    Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing (2002), Cell, 108, 637-648.
    View publication on PubMed
Search for more literature for 2.7.7.52

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, gene is located on chromosome 9, expression as His-tagged protein in Escherichia coli Leishmania tarentolae
DNA sequence determination, mitochondrial enzyme, expression as His-tagged protein in Escherichia coli Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
brRNA inhibits the number of added residues Leishmania tarentolae
additional information RNA interference, i.e. RNAi, induced by tetracyclines causes down-regulation of RET1 and RET2 expression in vitro and in vivo Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Trypanosoma brucei 5739
-
mitochondrion
-
 Leishmania tarentolae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Leishmania tarentolae
Mn2+ requires Mn2+ or Mg2+ Leishmania tarentolae
Mn2+ selectively inhibits the distributive activity of the enzyme Leishmania tarentolae
Mn2+ when GTP, CTP, or ATP are used as substrates, Mn2+ is more effective than Mg2+ Leishmania tarentolae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 native PAGE Leishmania tarentolae
500000
-
 native PAGE and gel filtration Trypanosoma brucei
700000
-
 RNA-stabilized higher complexed form, native PAGE and glycerol gradient sedimentation Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei
-
 ?
-
 ?
additional information Leishmania tarentolae enzyme interacts with a minor fraction of total RNA ligase ?
-
 ?
UTP + RNAn Trypanosoma brucei
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania tarentolae
-
-
-
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.67
-
 purified enzyme Leishmania tarentolae
8.2
-
 purified recombinant enzyme Leishmania tarentolae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn low activity Leishmania tarentolae diphosphate + RNAn+1
-
 ?
CTP + RNAn
-
 Leishmania tarentolae diphosphate + RNAn+1
-
 ?
GTP + RNAn about 50% of the activity with UTP, prefers Mn2+ as divalent cation Leishmania tarentolae diphosphate + RNAn+1
-
 ?
additional information
-
 Trypanosoma brucei ?
-
 ?
additional information enzyme interacts with a minor fraction of total RNA ligase Leishmania tarentolae ?
-
 ?
UTP + RNAn
-
 Trypanosoma brucei diphosphate + RNAn+1
-
 ?
UTP + RNAn RNA substrate specificity, overview Leishmania tarentolae diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
More enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily Trypanosoma brucei
More enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily Leishmania tarentolae