Crystallization (Comment) | Organism |
---|---|
structures of the complete heterodimer formed by polypeptides D1 and D12. The D1 subunit comprises an N-terminal RNA triphosphatase (TPase)-guanylyltransferase (GTase) module and a C-terminal guanine-N7-methyltransferase (MTase) module. The D12 subunit binds and allosterically stimulates the MTase module. An extensive TPase-GTase interface clamps the GTase nucleotidyltransferase and oligosaccharide-binding fold domains in a closed conformation around GTP | Vaccinia virus |
Protein Variants | Comment | Organism |
---|---|---|
E192A | 113% of wild-type guanylyltransferase activity, 4% of wild-type RNA triphosphatase activity | Vaccinia virus |
K478A | 1% of wild-type guanylyltransferase activity, 42% of wild-type RNA triphosphatase activity | Vaccinia virus |
L47A/L50A/T51A | 1% of wild-type guanylyltransferase activity, 43% of wild-type RNA triphosphatase activity | Vaccinia virus |
N181A | 48% of wild-type guanylyltransferase activity, 69% of wild-type RNA triphosphatase activity | Vaccinia virus |
R186A | 51% of wild-type guanylyltransferase activity, 92% of wild-type RNA triphosphatase activity | Vaccinia virus |
T10A | 19% of wild-type guanylyltransferase activity, 104% of wild-type RNA triphosphatase activity | Vaccinia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | P04298 | mRNA-capping enzyme catalytic subunit | - |
Vaccinia virus Western Reserve | P04298 | mRNA-capping enzyme catalytic subunit | - |
Synonyms | Comment | Organism |
---|---|---|
VACWR106 | - |
Vaccinia virus |