Crystallization (Comment) | Organism |
---|---|
crystals of RNA guanylyltransferase, i.e. capping enzyme, complexed with a mRNA cap analogue G(5')ppp(5')G, enzyme solution: 15 mg/ml, 1.3 mM GpppG, 50 mM Tris, pH 7.5, 0.4 M NaCl, 2 mM EDTA, 4 mM DTT, mixed with equal volume of equilibration solution: potassium phosphate 50 mM, pH 6.5, 5-10% PEG 8000, 2 mM ZnCl2, 24 h, X-ray structure determination and analysis | Paramecium bursaria Chlorella virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus 1 | - |
crystal structure: Protein Data Bank 1CKO | - |
Purification (Comment) | Organism |
---|---|
- |
Paramecium bursaria Chlorella virus 1 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mechanism of intermediate formation | Paramecium bursaria Chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | - |
Paramecium bursaria Chlorella virus 1 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? |
Synonyms | Comment | Organism |
---|---|---|
capping enzyme | - |
Paramecium bursaria Chlorella virus 1 |
mRNA guanylyl transferase | - |
Paramecium bursaria Chlorella virus 1 |