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Literature summary for 2.7.7.50 extracted from

  • Myette, J.R.; Niles, E.g.
    Domain structure of the vaccinia virus mRNA capping enzyme. Expression in Escherichia coli of a subdomain possessing the RNA 5'-triphosphatase and guanylyltransferase activities and a kinetic comparison to the full-size enzyme (1996), J. Biol. Chem., 271, 11936-11944.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant lacking the C-terminal domain, consisting of the D1R domain, residues 1-545, expression in Escherichia coli BL21(DE3)pLysS Vaccinia virus

Protein Variants

Protein Variants Comment Organism
additional information mutant consisting of the D1R domain, residues 1-545 Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
RNA pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme Vaccinia virus
0.0003
-
RNA pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 Vaccinia virus
0.013
-
GTP pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme Vaccinia virus
0.0155
-
GTP pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme C-terminally truncated to residues 1-545 Vaccinia virus

Reaction

Reaction Comment Organism Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA active domain between residues 520 and 545, comprises both activities, the ATPase and guanylyltransferase activity Vaccinia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0042
-
purified mutant, residues 1-545, D1R domain mutant, ATPase activity Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
Vaccinia virus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + RNA
-
Vaccinia virus ?
-
?

Synonyms

Synonyms Comment Organism
mRNA capping enzyme
-
Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Vaccinia virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0147
-
RNA pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 Vaccinia virus
0.0187
-
RNA pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme Vaccinia virus
0.0233
-
GTP pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme Vaccinia virus
0.0267
-
GTP pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Vaccinia virus