Cloned (Comment) | Organism |
---|---|
mutant lacking the C-terminal domain, consisting of the D1R domain, residues 1-545, expression in Escherichia coli BL21(DE3)pLysS | Vaccinia virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant consisting of the D1R domain, residues 1-545 | Vaccinia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
RNA | pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme | Vaccinia virus | |
0.0003 | - |
RNA | pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 | Vaccinia virus | |
0.013 | - |
GTP | pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme | Vaccinia virus | |
0.0155 | - |
GTP | pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 | Vaccinia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme C-terminally truncated to residues 1-545 | Vaccinia virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | active domain between residues 520 and 545, comprises both activities, the ATPase and guanylyltransferase activity | Vaccinia virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0042 | - |
purified mutant, residues 1-545, D1R domain mutant, ATPase activity | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | - |
Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + RNA | - |
Vaccinia virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mRNA capping enzyme | - |
Vaccinia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vaccinia virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0147 | - |
RNA | pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 | Vaccinia virus | |
0.0187 | - |
RNA | pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme | Vaccinia virus | |
0.0233 | - |
GTP | pH 8.0, 37°C, guanylyltransferase activity, holoenzyme capping enzyme | Vaccinia virus | |
0.0267 | - |
GTP | pH 8.0, 37°C, guanylyltransferase activity, D1R domain, residues 1-545 | Vaccinia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vaccinia virus |