Literature summary for 2.7.7.50 extracted from

Monroy, G.; Spencer, E.; Hurwitz, J.
Characteristics of reactions catalyzed by purified guanylyltransferase from vaccinia virus (1978), J. Biol. Chem., 253, 4490-4498.

Search for more literature for 2.7.7.50

Activating Compound

Activating Compound	Comment	Organism	Structure
bovine serum albumin	stimulates	Vaccinia virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0002	-	termini of 5'-triphosphate poly(A)	pH 7.8, 37°C	Vaccinia virus
0.017	-	GTP	pH 7.8, 37°C	Vaccinia virus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	can partially replace Mg2+ in activation	Vaccinia virus
K+	stimulates, maximal activation at 6 mM KCl	Vaccinia virus
Mg2+	required	Vaccinia virus
Mg2+	maximal activity at 0.5-2 mM	Vaccinia virus
Mn2+	can partially replace Mg2+ in activation	Vaccinia virus
Na+	stimulates, maximal activation at 50-75 mM NaCl	Vaccinia virus

Organism

Organism	UniProt	Comment	Textmining
Vaccinia virus	-	-	-

Storage Stability

Storage Stability	Organism
0°C, 48 h, 90% loss of activity, 59000 MW protein which lacks 7-methyltransferase activity	Vaccinia virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + ppp(5')RNA	specifically requires 5'-triphosphate-terminated RNA chains	Vaccinia virus	G(5')pppp(5')RNA + diphosphate	-	r
GTP + termini of 5'-triphosphate poly(A)	-	Vaccinia virus	?	-	?
pppG + ppp(5')A(pA)n	-	Vaccinia virus	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Vaccinia virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Vaccinia virus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)