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Literature summary for 2.7.7.50 extracted from
- Purification and characterization of mRNA guanylyltransferase from HeLa cell nuclei (1980), J. Biol. Chem., 255, 2829-2834.
General Stability
| General Stability | Organism |
|---|---|
| stable after a few cycles of freezing and thawing | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 2-5 mM | Homo sapiens |  |
| Mg2+ | can partially replace Mn2+ in activation | Homo sapiens | |
| Mn2+ | required | Homo sapiens | |
| Mn2+ | maximal activity at 2 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48500 | - |
sucrose density gradient sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
HeLa cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000066 | - |
- |
Homo sapiens |
| 0.00007 | - |
purified enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 9 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + pp(5')RNA | - |
Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 45% of activity maximum, pH 8.5: about 15% of activity maximum | Homo sapiens |
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Release 2023.1 (January 2023)
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