Any feedback?
Literature summary for 2.7.7.5 extracted from
- "ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases (2000), J. Biol. Chem., 275, 1691-1698.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thiobacillus denitrificans |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme stabilized by 5 mg/ml bovine serum albumin | Thiobacillus denitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thiobacillus denitrificans |  |
| diacetyl | slow but complete inactivation | Thiobacillus denitrificans | |
| diethyldicarbonate | - |
Thiobacillus denitrificans | |
| Phenylglyoxal | incomplete inactivation | Thiobacillus denitrificans | |
| sulfate | inhibitory at high concentrations | Thiobacillus denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
adenosine 5'-phosphosulfate | pH 7.6 | Thiobacillus denitrificans | |
| 12 | - |
phosphate | pH 7.6 | Thiobacillus denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no effect | Thiobacillus denitrificans | |
| Cu2+ | no effect | Thiobacillus denitrificans | |
| Fe2+ | very slight inhibition | Thiobacillus denitrificans | |
| Mn2+ | no effect | Thiobacillus denitrificans | |
| Zn2+ | very slight inhibition | Thiobacillus denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE | Thiobacillus denitrificans |
| 41380 | - |
MALDI-MS | Thiobacillus denitrificans |
| 41390 | - |
calculated from DNA sequence | Thiobacillus denitrificans |
| 82000 | - |
gel filtration | Thiobacillus denitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + adenosine 5'-phosphosulfate | Thiobacillus denitrificans | - |
ADP + SO42- | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | Thiobacillus denitrificans RT | - |
ADP + SO42- | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thiobacillus denitrificans | Q9LA72 | - |
- |
| Thiobacillus denitrificans RT | Q9LA72 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thiobacillus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2100 | - |
purified enzyme, pH 7.6 | Thiobacillus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with ADP, CDP, GDP, UDP and IDP | Thiobacillus denitrificans | ? | - |
? | |
| additional information | no activity with ADP, CDP, GDP, UDP and IDP | Thiobacillus denitrificans RT | ? | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | - |
Thiobacillus denitrificans | ADP + SO42- | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | ping pong reaction mechanism | Thiobacillus denitrificans | ADP + SO42- | - |
ir | |
| phosphate + adenosine 5'-phosphosulfate | highly specific for adenosine 5'-phosphosulfate | Thiobacillus denitrificans | ADP + SO42- | - |
ir | |
| phosphate + adenosine 5'-phosphosulfate | - |
Thiobacillus denitrificans RT | ADP + SO42- | - |
? | |
| phosphate + adenosine 5'-phosphosulfate | ping pong reaction mechanism | Thiobacillus denitrificans RT | ADP + SO42- | - |
ir | |
| phosphate + adenosine 5'-phosphosulfate | highly specific for adenosine 5'-phosphosulfate | Thiobacillus denitrificans RT | ADP + SO42- | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41000, gel filtration, SDS-PAGE | Thiobacillus denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the name sulfurylase should not be used because the reaction was found to be irreversible, enzyme catalyzes the formation of ADP and SO42- from phosphate and adenosine 5'-phosphosulfate | Thiobacillus denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Thiobacillus denitrificans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
