Literature summary for 2.7.7.49 extracted from

Konishi, A.; Hisayoshi, T.; Yokokawa, K.; Barrioluengo, V.; Menendez-Arias, L.; Yasukawa, K.
Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation (2014), Biochem. Biophys. Res. Commun., 454, 269-274.

Search for more literature for 2.7.7.49

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Moloney murine leukemia virus

Protein Variants

Protein Variants	Comment	Organism
D524A	mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes	Moloney murine leukemia virus
E286R	mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes	Moloney murine leukemia virus
E286R/E302K/L435R/D524A	mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes	Moloney murine leukemia virus
E302K	mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes	Moloney murine leukemia virus
L435R	mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes	Moloney murine leukemia virus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Moloney murine leukemia virus	-	-	-

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Release 2023.1 (January 2023)