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Literature summary for 2.7.7.49 extracted from
- Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4 (2012), J. Biosci. Bioeng., 113, 315-321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic tree, expression of K4PolI in Escherichia coli | Thermotoga petrophila |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F388A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| L329A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| L329A/Q384A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| L329A/Y438A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| M408A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| Q384A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| Q384A/Y438A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| T326A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
| Y438A | site-directed mutagenesis, the mutant exhibits DNA-dependent and RNA-dependent DNA polymerase activities, while the wild-type enzyme is a DNA-depedent DNA polymerase | Thermotoga petrophila |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Thermotoga petrophila |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Thermotoga petrophila | - |
diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Thermotoga petrophila K4 | - |
diphosphate + DNAn+1 | - |
? | |
| additional information | Thermotoga petrophila | DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49 | ? | - |
? | |
| additional information | Thermotoga petrophila K4 | DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga petrophila | - |
- |
- |
| Thermotoga petrophila K4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant K4PolI from Escherichia coli | Thermotoga petrophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quantitative analysis of DNA- and RNA-dependent DNA polymerase activity of enzyme mutants, overview | Thermotoga petrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Thermotoga petrophila | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | RNA-dependent DNA polymerase activity by enzyme mutants T326A, L329A, Q384A, F388A, M408A, Y438A, L329A/Q384A, L329A/Y438A, and Q384A/Y438A, not by the wild-type enzyme | Thermotoga petrophila | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Thermotoga petrophila K4 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | RNA-dependent DNA polymerase activity by enzyme mutants T326A, L329A, Q384A, F388A, M408A, Y438A, L329A/Q384A, L329A/Y438A, and Q384A/Y438A, not by the wild-type enzyme | Thermotoga petrophila K4 | diphosphate + DNAn+1 | - |
? | |
| additional information | DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49 | Thermotoga petrophila | ? | - |
? | |
| additional information | DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49 | Thermotoga petrophila K4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure modeling of K4 polymerase, overview | Thermotoga petrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| K4 polymerase | - |
Thermotoga petrophila |
| K4PolI | - |
Thermotoga petrophila |
| reverse transcriptase | - |
Thermotoga petrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | assay at | Thermotoga petrophila |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | K4PolI is a family A DNA polymerase, phylogenetic tree | Thermotoga petrophila |
| additional information | structure modeling of K4 polymerase, overview | Thermotoga petrophila |
| physiological function | reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase | Thermotoga petrophila |
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