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Literature summary for 2.7.7.49 extracted from
- Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases (2012), Biochem. Biophys. Res. Commun., 427, 654-658.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Moloney murine leukemia virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Moloney murine leukemia virus | |
| 0.0056 | - |
DNAn | pH 8.2, 37°C, recombinant enzyme, substrate is poly(rA)-p(dT)45 | Moloney murine leukemia virus |  |
| 0.25 | - |
dTTP | pH 8.2, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Moloney murine leukemia virus | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moloney murine leukemia virus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| dTTP + DNAn | incorporation of dTTP into poly(rA)-p(dT)45 | Moloney murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MMLV RT | - |
Moloney murine leukemia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Moloney murine leukemia virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29 | - |
DNAn | pH 8.2, 37°C, recombinant enzyme, substrate is poly(rA)-p(dT)45 | Moloney murine leukemia virus | |
| 35 | - |
dTTP | pH 8.2, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Moloney murine leukemia virus |
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Release 2023.1 (January 2023)
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