Protein Variants | Comment | Organism |
---|---|---|
D67N/K70R/T215Y/K219Q | mutation results in a 1.5fold decrease in the rate constant for polymerization and a 2.5fold decrease in the equilibrium dissociation constant for 3'-azido-3'-deoxythymidine 5'-triphosphate compared to wild-type enzyme. These values translate into a 4fold decrease in selectivity for 3'-azido-3'-deoxythymidine 5'-monophosphate incorporation ba the mutant enzyme as compared to wild-type enzyme for RNA dependent DNA replication. No such decrease in selectivity is detected for DNA dependent replication | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avian myeloblastosis virus | - |
wild-type and 3'-azido-3'-deoxythymidine resistant strain D67N/K70R/T215Y/K219Q | - |
Human immunodeficiency virus 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and 3'-azido-3'-deoxythymidine resistant strain D67N/K70R/T215Y/K219Q, expression in Escherichia coli | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Avian myeloblastosis virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Human immunodeficiency virus 1 | diphosphate + DNAn+1 | - |
? |