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Literature summary for 2.7.7.49 extracted from
- Simian immunodeficiency virus reverse transcriptase. Purification and partial characterization (1990), Eur. J. Biochem., 192, 207-213.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ddATP | competitive with respect to dATP, noncompetitive with respect to dCTP, dGTP and dTTP | Simian immunodeficiency virus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
dTTP | pH 7.6, 37°C | Simian immunodeficiency virus | |
| 0.003 | - |
dATP | pH 7.6, 37°C | Simian immunodeficiency virus | |
| 0.0047 | - |
dCTP | pH 7.6, 37°C | Simian immunodeficiency virus | |
| 0.0055 | - |
dGTP | pH 7.6, 37°C | Simian immunodeficiency virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Simian immunodeficiency virus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.067 | - |
- |
Simian immunodeficiency virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 6 months | Simian immunodeficiency virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + DNAn | - |
Simian immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dCTP + DNAn | - |
Simian immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | the enzyme shows both RNA-dependent and DNA-dependent DNA synthesis activity and an associated RNAse H activity | Simian immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dGTP + DNAn | - |
Simian immunodeficiency virus | diphosphate + DNAn+1 | - |
? | |
| dTTP + poly(rA)/(dT)18 | - |
Simian immunodeficiency virus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | - |
Simian immunodeficiency virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.8 | pH 7.0: about 40% of maximal activity, pH 8.8: about 75% of maximal activity | Simian immunodeficiency virus |
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