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Literature summary for 2.7.7.49 extracted from

  • Leis, J.; Hurwitz, J.
    RNA-dependent DNA polymerase from avian myeloblastosis virus (1974), Methods Enzymol., 29E, 143-150.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol sulfhydryl reagents required for optimal activity: dithiothreitol or 2-mercaptoethanol Avian myeloblastosis virus

General Stability

General Stability Organism
presence of 0.2 M ammonium sulfate helps to stabilize the enzyme Avian myeloblastosis virus

Inhibitors

Inhibitors Comment Organism Structure
KCl 80 mM, 50% inhibition Avian myeloblastosis virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for deoxynucleoside triphosphates is 0.01-0.03 mM Avian myeloblastosis virus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5 mM, stimulates rate of DNA synthesis by 20% Avian myeloblastosis virus
Mg2+ requires Mg2+ for activity with DNA or AMV RMA. Optimal activity at 10 mM Avian myeloblastosis virus
Mn2+ can partially replace Mg2+ Avian myeloblastosis virus

Organism

Organism UniProt Comment Textmining
Avian myeloblastosis virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Avian myeloblastosis virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000104
-
above Avian myeloblastosis virus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 2 mM dithioerythritol, less than 20% loss of activity after 2 months Avian myeloblastosis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the polymerase requires a primer strand with free 3'-hydroxyl group and a template strand to direct DNA synthesis Avian myeloblastosis virus diphosphate + DNAn+1
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
at high glycerol concehntrations, 2.5 min, 50% loss of activity Avian myeloblastosis virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Avian myeloblastosis virus

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.8 about 50% of maximal activity at pH 7.2 and pH 9.8 Avian myeloblastosis virus