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Literature summary for 2.7.7.48 extracted from

  • Dangerfield, T.L.; Huang, N.Z.; Johnson, K.A.
    Remdesivir is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase (2020), iScience, 23, 101849 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of SARS-CoV-2 RNA-dependent RNA polymerase subunits with chaperones gives soluble expression in Escherichia coli Severe acute respiratory syndrome coronavirus 2

Inhibitors

Inhibitors Comment Organism Structure
remdesivir triphosphate effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase Severe acute respiratory syndrome coronavirus 2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
0.13
-
 UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
0.32
-
 ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Severe acute respiratory syndrome coronavirus 2
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome coronavirus 2 P0DTD1 replicase polyprotein 1ab; SARS-CoV-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Severe acute respiratory syndrome coronavirus 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn
-
 Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn
-
 Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
 ?
remdesivir triphosphate + RNAn remdesivir triphosphate is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
 ?
UTP + RNAn
-
 Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
RDRP
-
 Severe acute respiratory syndrome coronavirus 2
RNA-dependent RNA polymerase
-
 Severe acute respiratory syndrome coronavirus 2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
240
-
 ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
308
-
 UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
680
-
 remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00074
-
 ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
0.00129
-
 remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
0.0023
-
 UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2