Literature summary for 2.7.7.48 extracted from

Peng, Q.; Peng, R.; Yuan, B.; Zhao, J.; Wang, M.; Wang, X.; Wang, Q.; Sun, Y.; Fan, Z.; Qi, J.; Gao, G.F.; Shi, Y.
Structural and biochemical characterization of the nsp12-nsp7-nsp8 core polymerase complex from SARS-CoV-2 (2020), Cell Rep., 31, 107774 .

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Cloned(Commentary)

Cloned (Comment)	Organism
SARS-CoV-2 nsp12 polymerase and nsp7-nsp8 cofactors are expressed using the baculovirus and Escherichia coli expression systems, respectively. The three protein subunits were mixed in vitro to constitute the core polymerase complex	Severe acute respiratory syndrome coronavirus 2

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nucleoside triphosphate + RNAn	Severe acute respiratory syndrome coronavirus 2	-	diphosphate + RNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Severe acute respiratory syndrome coronavirus 2	P0DTD1	replicase polyprotein 1ab; SARS-CoV-2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nucleoside triphosphate + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	?
nucleoside triphosphate + RNAn	SARS-CoV-2 core polymerase complex has less efficient activity for RNA synthesis and lower thermostability of individual subunits compared with SARSCoV	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	?

Subunits

Subunits	Comment	Organism
More	cryo-EM structure of SARS-CoV-2 nsp12-nsp7-nsp8 core polymerase complex. The structure of the SARS-CoV-2 nsp12-nsp7-nsp8 complex is determined at 3.7-A resolution. The SARSCoV-2 polymerase complex consists of the nsp12 catalytic subunit and nsp7-nsp8 cofactors	Severe acute respiratory syndrome coronavirus 2

Synonyms

Synonyms	Comment	Organism
RDRP	-	Severe acute respiratory syndrome coronavirus 2
SARSCoV-2-core polymerase complex	-	Severe acute respiratory syndrome coronavirus 2

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	lower thermostability of individual nsp7-8-12 subunits of SARS-CoV-2 core polymerase complex compared with SARS-CoV core polymerase complex	Severe acute respiratory syndrome coronavirus 2

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Release 2023.1 (January 2023)