Cloned (Comment) | Organism |
---|---|
recombinant expression of FLAG-tagged full-length enzyme in HEK-293 cells | Enterovirus A71 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Enterovirus A71 | 5737 | - |
nucleus | enzyme 3Dpol and Prp8 are colocalized in the nucleus | Enterovirus A71 | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Enterovirus A71 | cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with pre-mRNA processing factor 8, Prp8. Identification of potential protein targets of EV71 3Dpol by MALDI-TOF MS analysis, overview | ? | - |
? | |
additional information | Enterovirus A71 40 | cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with pre-mRNA processing factor 8, Prp8. Identification of potential protein targets of EV71 3Dpol by MALDI-TOF MS analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus A71 | Q2XTR4 | a picornavirus, harvested from MOI-infected RD cells | - |
Enterovirus A71 40 | Q2XTR4 | a picornavirus, harvested from MOI-infected RD cells | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with pre-mRNA processing factor 8, Prp8. Identification of potential protein targets of EV71 3Dpol by MALDI-TOF MS analysis, overview | Enterovirus A71 | ? | - |
? | |
additional information | interaction of EV71 3Dpol and the nuclear protein U5 snRNPs. ENzyme 3Dpol enters the cellular nucleus and colocalizes with pre-mRNA processing factor 8, Prp8, 3Dpol associates with the C-terminal domain of Prp8 containing the Jab1/MPN region. RIP-seq of the pre-mRNA are trapped by the Prp8-3Dpol complexes | Enterovirus A71 | ? | - |
? | |
additional information | cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with pre-mRNA processing factor 8, Prp8. Identification of potential protein targets of EV71 3Dpol by MALDI-TOF MS analysis, overview | Enterovirus A71 40 | ? | - |
? | |
additional information | interaction of EV71 3Dpol and the nuclear protein U5 snRNPs. ENzyme 3Dpol enters the cellular nucleus and colocalizes with pre-mRNA processing factor 8, Prp8, 3Dpol associates with the C-terminal domain of Prp8 containing the Jab1/MPN region. RIP-seq of the pre-mRNA are trapped by the Prp8-3Dpol complexes | Enterovirus A71 40 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3Dpol | - |
Enterovirus A71 |
picornaviral 3D polymerase | - |
Enterovirus A71 |
RDRP | - |
Enterovirus A71 |
General Information | Comment | Organism |
---|---|---|
physiological function | mechanism of viruses attacking hosts whereby picornaviral 3D polymerase (3Dpol) enters the nucleus and targets the central pre-mRNA processing factor 8 (Prp8) to block premRNA splicing and mRNA synthesis. The 3Dpol inhibits the second catalytic step of the splicing process, resulting in the accumulation of the lariat-form and the reduction of the mRNA. 3Dpol inhibits intracellular pre-mRNA splicing by interacting with Prp8, modelling, overview | Enterovirus A71 |