Cloned (Comment) | Organism |
---|---|
expression of the recombinant enzyme in Escherichia coli | influenza A virus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, at 20°C by the hanging drop method using a protein solution of 5-10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl and 2.5 mM MnCl2 and a reservoir composition of 1.2 M Li2SO4, 100 mM MES, pH 6.0, 10 mM Mg acetate and 3% ethylene glycol | influenza A virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | influenza A virus | - |
diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | influenza A virus Victoria/3/1975 H3N2 | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
influenza A virus | P31343 | subunit P2; Victoria/3/1975, H3N2 | - |
influenza A virus Victoria/3/1975 H3N2 | P31343 | subunit P2; Victoria/3/1975, H3N2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by affinity chromatography and gel filtration | influenza A virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the polymerase protein also harbors an intrinsic RNA and DNA endonuclease activity that cleaves host mRNAs during cap-snatching, inhibited by 2,4-dioxo-4-phenylbutanoic and activated by Mn2+, with the amino-terminal 209 residues of the PA subunit containing the endonuclease active site, structure-function analysis, overview | influenza A virus | ? | - |
? | |
additional information | the polymerase protein also harbors an intrinsic RNA and DNA endonuclease activity that cleaves host mRNAs during cap-snatching, inhibited by 2,4-dioxo-4-phenylbutanoic and activated by Mn2+, with the amino-terminal 209 residues of the PA subunit containing the endonuclease active site, structure-function analysis, overview | influenza A virus Victoria/3/1975 H3N2 | ? | - |
? | |
nucleoside triphosphate + RNAn | - |
influenza A virus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | various RNA substrates: Alu RNA, 110 nucleotides of the Alu domain of Pyrococcus horikoshii SRP RNA, Candida albicans tRNAAsn, U-rich RNA (59-GGCCAUCCUGU7 CCCU11CU19-39)29, ph-RNA of 81 nucleotides30, and short ph-RNA of 36 nucleotides comprising just the conserved 3' and 5' ends with a short linker and circular single stranded DNA | influenza A virus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
influenza A virus Victoria/3/1975 H3N2 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | various RNA substrates: Alu RNA, 110 nucleotides of the Alu domain of Pyrococcus horikoshii SRP RNA, Candida albicans tRNAAsn, U-rich RNA (59-GGCCAUCCUGU7 CCCU11CU19-39)29, ph-RNA of 81 nucleotides30, and short ph-RNA of 36 nucleotides comprising just the conserved 3' and 5' ends with a short linker and circular single stranded DNA | influenza A virus Victoria/3/1975 H3N2 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | composed of three subunits, PA, PB1 and PB2 | influenza A virus |
Synonyms | Comment | Organism |
---|---|---|
influenza polymerase PA | - |
influenza A virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | influenza A virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | influenza A virus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is responsible for replication and transcription of the eight separate segments of the viral RNA genome in the nuclei of infected cells | influenza A virus |