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Literature summary for 2.7.7.48 extracted from

  • Hass, M.; Lelke, M.; Busch, C.; Becker-Ziaja, B.; Guenther, S.
    Mutational evidence for a structural model of the Lassa virus RNA polymerase domain and identification of two residues, Gly1394 and Asp1395, that are critical for transcription but not replication of the genome (2008), J. Virol., 82, 10207-10217.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons, expression of mutant enzymes in BHK cells Lassa mammarenavirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lassa mammarenavirus the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of thedomain, overview ?
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Organism

Organism UniProt Comment Textmining
Lassa mammarenavirus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of thedomain, overview Lassa mammarenavirus ?
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 ?
additional information residues K1127, R1134, E1135, L1136, D1140, and K1144, D1193, G1298, S1333, D1334, D1335, and K1376 from different subunits might be involved in catalysis Lassa mammarenavirus ?
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Subunits

Subunits Comment Organism
More the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of the domain, overview Lassa mammarenavirus

Synonyms

Synonyms Comment Organism
RDRP
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 Lassa mammarenavirus
RNA polymerase
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 Lassa mammarenavirus
RNA-dependent RNA polymerase
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 Lassa mammarenavirus

Expression

Organism Comment Expression
Lassa mammarenavirus large-scale mutagenesis of the RdRp domain of Lassa virus L protein, overview. Mutation of two adjacent residues in the putative palm-thumb subdomain junction, G1394 and D1395, by PCR-based mutagenesis in strain AV, lead to a defect in mRNA synthesis but do not affect antigenomic RNA synthesis. Clear dominant-negative effects are only seen with mutants affecting residues in motifs pre-A with K1127, R1134, E1135, L1136, D1140, and K1144, A with D1193, B with G1298, C with S1333, D1334, and D1335, and D with K1376, overview additional information