Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, NS5B genotyping, enzyme activities, and activity with ribavirin, overview. Expression of His-tagged enzyme variants in Escherichia coli | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | P26663 | i.e. HCV | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme variants from Escherichia coli by nickel affinity chromatography | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
ribavirin triphosphate + RNAn | ribavirin is a guanosine analogue that can be a substrate for the viral RNA polymerase. HCV is genetically variable, and this genetic variation can affect the polymerase's use of ribavirin triphosphate, overview | Hepacivirus C | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS5B | - |
Hepacivirus C |
RDRP | - |
Hepacivirus C |
RNA polymerase | - |
Hepacivirus C |