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Literature summary for 2.7.7.48 extracted from

  • Zhang, S.; Weng, L.; Geng, L.; Wang, J.; Zhou, J.; Deubel, V.; Buchy, P.; Toyoda, T.
    Biochemical and kinetic analysis of the influenza virus RNA polymerase purified from insect cells (2009), Biochem. Biophys. Res. Commun., 391, 570-574.
    View publication on PubMed
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the viral His18-tagged enzyme components in Sf21AE and TN5 insect cells, establishing a single-round transcription system influenza A virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of the recombinant enzyme complex influenza A virus
0.0027
-
 CTP pH 8.0, 25°C, HCV JFH1a de novo transcription influenza A virus
0.0047
-
 ATP pH 8.0, 25°C, HCV JFH1a de novo transcription influenza A virus
0.0075
-
 UTP pH 8.0, 25°C, HCV JFH1a de novo transcription influenza A virus
0.0107
-
 GTP pH 8.0, 25°C, Influenza ApG-primed transcription influenza A virus
0.022
-
 UTP pH 8.0, 25°C, Influenza ApG-primed transcription influenza A virus
0.0289
-
 CTP pH 8.0, 25°C, Influenza ApG-primed transcription influenza A virus
0.0332
-
 ATP pH 8.0, 25°C, Influenza ApG-primed transcription influenza A virus
0.087
-
 GTP pH 8.0, 25°C, HCV JFH1a de novo transcription influenza A virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates optimally at 8 mM, recombinant viral enzyme purified from insect cells influenza A virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn influenza A virus
-
 diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
influenza A virus
-
 from insect cells
-

Purification (Commentary)

Purification (Comment) Organism
recombinant viral His18-tagged enzyme components from Sf21AE and TN5 insect cells influenza A virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?
CTP + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?
GTP + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?
additional information the RdRp catalyzes all the biochemical reactions of influenza virus transcription and replication in vitro, dinucleotide ApG and globin mRNA-primed transcription, de novo initiation/replication, and polyadenylation influenza A virus ?
-
 ?
nucleoside triphosphate + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn in vitro transcription using the model RNA template, v84 influenza A virus diphosphate + RNAn+1
-
 ?
UTP + RNAn
-
 influenza A virus diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
RDRP
-
 influenza A virus
RNA polymerase
-
 influenza A virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 recombinant viral enzyme purified from insect cells influenza A virus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 42 28.7% at 4°C and 13.4% at 42°C of the activity at 25°C, no activity at 0°C influenza A virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 wild-type enzyme, broad optimum influenza A virus
8
-
 recombinant viral enzyme purified from insect cells influenza A virus