Literature summary for 2.7.7.48 extracted from

Freistadt, M.S.; Vaccaro, J.A.; Eberle, K.E.
Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase (2007), Virol. J., 4, 44.

Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pCG1 cells	Enterovirus C

Inhibitors

Inhibitors	Comment	Organism	Structure
heparin	100 nM heparin rapidly inactivates 3Dpol by inhibiting reinitiation	Enterovirus C
NTP	3Dpol is inhibited by NTP concentrations higher than 2 mM	Enterovirus C

Organism

Organism	UniProt	Comment	Textmining
Enterovirus C	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose SPHP XK16/20 column chromatography, Q20 column chromatography, Q5 column chromatography, and UNO column chromatography	Enterovirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nucleoside triphosphate + RNAn	-	Enterovirus C	diphosphate + RNAn+1	-	?
nucleoside triphosphate + RNAn	RdRP incorporation of incorrect nucleosides is inefficient, making precise determination of kinetic parameters experimentally challenging. The fidelity for poliovirus polymerase 3Dpol ranges from 12000 to 1000000 for transition mutations and 32000 to 43000000 for transversion mutations	Enterovirus C	diphosphate + RNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
3Dpol	-	Enterovirus C
polymerase 3Dpol	-	Enterovirus C
RDRP	-	Enterovirus C
RNA-dependent RNA polymerase	-	Enterovirus C

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)