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Literature summary for 2.7.7.48 extracted from

  • Donofrio, J.C.; Kuchta, J.; Moore, R.; Kaczmarczyk, W.
    Properties of a solubilized replicase isolated from corn infected with Maize dwarf mosaic virus (1986), Can. J. Microbiol., 32, 637-644.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
RNA 2-10fold stimulation with the addition of various RNA templates. CCMV and CPMV RNA produces the greatest stimulation. TMV RNA also acts as a template, but with much less efficiency Maize dwarf mosaic virus
Sodium diethyldithiocarbamate 5 mM, great stimulation of template-dependent activity and to a lesser extent template-independent activity Maize dwarf mosaic virus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 12.5 mM, replicase activity is reduced by 50% Maize dwarf mosaic virus
RNase completely eliminates incorporation Maize dwarf mosaic virus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme is associated with particulate fraction of Zea mays leaf infected with Maize dwarf mosaic virus Maize dwarf mosaic virus
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 2.8 mM Maize dwarf mosaic virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
glycerol density gradient sedimentation Maize dwarf mosaic virus

Organism

Organism UniProt Comment Textmining
Maize dwarf mosaic virus
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Zea mays infected with
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Source Tissue

Source Tissue Comment Organism Textmining
additional information leaf of Zea mays infected with Maize dwarf mosaic virus Maize dwarf mosaic virus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
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Maize dwarf mosaic virus diphosphate + RNAn+1
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?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
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-
Maize dwarf mosaic virus

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9.7 pH 6.3: about 45% of maximal activity, pH 9.7: about 35% of maximal activity Maize dwarf mosaic virus