Crystallization (Comment) | Organism |
---|---|
wild type enzyme complexed with ATP, magnesium, and streptomycin, sitting drop vapor diffusion method, using 10% (w/v) PEG 20,000, 20% (v/v) PEG monomethyl ether 550, 0.1 M MOPS/HEPES-Na, pH 7.5. Mutant enzyme E87Q complexed with ATP and streptomycin or dihydrostreptomycin, sitting drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.2 M CaCl2, and 0.1 M HEPES, pH 7.5 | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
D178A | the mutation destabilizes the enzyme leading to reduced melting temperature (47 C). When titrated with streptomycin, temperature stabilization occurs at higher streptomycin concentration compared with wild type, indicating weaker binding affinity | Salmonella enterica |
E87Q | the nonadenylating mutant can hydrolyze ATP in the presence of streptomycin. The mutant does not convey resistance to streptomycin but still binds ATP and streptomycin, although with 4 and 20fold lower affinity than the wild type enzyme | Salmonella enterica |
W173A | the mutation destabilizes the enzyme leading to reduced melting temperature (50 C). When titrated with streptomycin, temperature stabilization occurs at higher streptomycin concentration compared with wild type, indicating weaker binding affinity | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + spectinomycin | Salmonella enterica | - |
diphosphate + 9-adenylylspectinomycin | - |
? | |
ATP + streptomycin | Salmonella enterica | - |
diphosphate + 3''-adenylylstreptomycin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + spectinomycin | - |
Salmonella enterica | diphosphate + 9-adenylylspectinomycin | - |
? | |
ATP + streptomycin | - |
Salmonella enterica | diphosphate + 3''-adenylylstreptomycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aadA | - |
Salmonella enterica |
aminoglycoside (3'')(9) adenylyltransferase | - |
Salmonella enterica |
aminoglycoside adenylyltransferase | - |
Salmonella enterica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
melting temperature | Salmonella enterica |