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Literature summary for 2.7.7.47 extracted from

  • Stern, A.L.; Van der Verren, S.E.; Kanchugal P, S.; Naesvall, J.; Gutierrez-de-Teran, H.; Selmer, M.
    Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica (2018), J. Biol. Chem., 293, 11481-11490 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type enzyme complexed with ATP, magnesium, and streptomycin, sitting drop vapor diffusion method, using 10% (w/v) PEG 20,000, 20% (v/v) PEG monomethyl ether 550, 0.1 M MOPS/HEPES-Na, pH 7.5. Mutant enzyme E87Q complexed with ATP and streptomycin or dihydrostreptomycin, sitting drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.2 M CaCl2, and 0.1 M HEPES, pH 7.5 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
D178A the mutation destabilizes the enzyme leading to reduced melting temperature (47 C). When titrated with streptomycin, temperature stabilization occurs at higher streptomycin concentration compared with wild type, indicating weaker binding affinity Salmonella enterica
E87Q the nonadenylating mutant can hydrolyze ATP in the presence of streptomycin. The mutant does not convey resistance to streptomycin but still binds ATP and streptomycin, although with 4 and 20fold lower affinity than the wild type enzyme Salmonella enterica
W173A the mutation destabilizes the enzyme leading to reduced melting temperature (50 C). When titrated with streptomycin, temperature stabilization occurs at higher streptomycin concentration compared with wild type, indicating weaker binding affinity Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + spectinomycin Salmonella enterica
-
diphosphate + 9-adenylylspectinomycin
-
?
ATP + streptomycin Salmonella enterica
-
diphosphate + 3''-adenylylstreptomycin
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + spectinomycin
-
Salmonella enterica diphosphate + 9-adenylylspectinomycin
-
?
ATP + streptomycin
-
Salmonella enterica diphosphate + 3''-adenylylstreptomycin
-
?

Synonyms

Synonyms Comment Organism
aadA
-
Salmonella enterica
aminoglycoside (3'')(9) adenylyltransferase
-
Salmonella enterica
aminoglycoside adenylyltransferase
-
Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
melting temperature Salmonella enterica