KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
streptomycin | pH 7.8, 37°C | Bacillus subtilis | |
0.00032 | - |
streptomycin | pH 7.8, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromosome | - |
Bacillus subtilis | 5694 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
- |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + streptomycin | Bacillus subtilis | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | Escherichia coli | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | Bacillus subtilis Marburg168 BD224 | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | diphosphate + 3''-adenylylstreptomycin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
Marburg168 BD224 | - |
Bacillus subtilis Marburg168 BD224 | - |
Marburg168 BD224 | - |
Escherichia coli | - |
K12 W1895 HfrC | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000046 | - |
streptomycin | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + streptomycin | - |
Bacillus subtilis | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | - |
Escherichia coli | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | Bacillus subtilis | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | Escherichia coli | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | - |
Bacillus subtilis Marburg168 BD224 | diphosphate + 3''-adenylylstreptomycin | - |
? | |
ATP + streptomycin | enzyme leads to resistance, destroys the inhibitory activity of streptomycin | Bacillus subtilis Marburg168 BD224 | diphosphate + 3''-adenylylstreptomycin | - |
? | |
additional information | does not adenylate spectinomycin, enzyme might be identical to AAD aminoglycoside 3''-adenylyltransferase | Bacillus subtilis | ? | - |
? | |
additional information | does not adenylate spectinomycin, enzyme might be identical to AAD aminoglycoside 3''-adenylyltransferase | Bacillus subtilis Marburg168 BD224 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus subtilis |
50 | - |
- |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
9 | - |
- |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
5.2 |
Bacillus subtilis | isoelectric focusing | - |
6 |