Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D44A | the mutation causes a 4fold decrease in aminoglycoside adenylation compared to the wild type enzyme | Klebsiella pneumoniae |
I129A | the mutation causes a 3fold decrease in aminoglycoside adenylation compared to the wild type enzyme | Klebsiella pneumoniae |
Y135A | the mutation causes a 1.6fold decrease in aminoglycoside adenylation compared to the wild type enzyme | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 100 mM used in assay conditions | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | P0AE05 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gentamicin | - |
Klebsiella pneumoniae | diphosphate + 2''-adenylylgentamicin | - |
? | |
ATP + kanamycin A | - |
Klebsiella pneumoniae | diphosphate + 2''-adenylylkanamycin A | - |
? | |
ATP + kanamycin B | - |
Klebsiella pneumoniae | diphosphate + 2''-adenylylkanamycin B | - |
? | |
ATP + tobramycin A | - |
Klebsiella pneumoniae | diphosphate + 2''-adenylyltobramycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside adenyltransferase 2''-Ia | - |
Klebsiella pneumoniae |
ANT(2'')-Ia | - |
Klebsiella pneumoniae |