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Literature summary for 2.7.7.46 extracted from
- Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa (1982), J. Antibiot., 35, 235-244.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | gentamicin resistance, mediated by multiple resistance plasmids in different species of Enterobacteriaceae, can be a problem in hospitals | Pseudomonas aeruginosa |
General Stability
| General Stability | Organism |
|---|---|
| highly unstable after purification | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | above 0.5 mM | Pseudomonas aeruginosa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM | Pseudomonas aeruginosa | |
| Mg2+ | shows enzymatic activity only in presence of cations, optimal concentration 2.0 mM | Pseudomonas aeruginosa | |
| additional information | K+ and Na+ have almost no effect, NH4+ has a low enhancing effect | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gentamicin A | Pseudomonas aeruginosa | gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors | diphosphate + 2''-adenylylgentamicin A | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| plasmid pPK237, purified from an E. coli K12 host RC85F- | Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, activity remains only for a few hours | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dibekacin | - |
Pseudomonas aeruginosa | diphosphate + 2''-adenylyldibekacin | - |
r | |
| ATP + gentamicin A | gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors | Pseudomonas aeruginosa | diphosphate + 2''-adenylylgentamicin A | - |
r | |
| ATP + gentamicin C1 | gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors | Pseudomonas aeruginosa | diphosphate + 2''-adenylylgentamicin C1 | - |
? | |
| ATP + gentamicin C1a | gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors | Pseudomonas aeruginosa | diphosphate + 2''-adenylylgentamicin C1a | - |
? | |
| ATP + gentamicin C2 | gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors | Pseudomonas aeruginosa | diphosphate + 2''-adenylylgentamicin C2 | - |
? | |
| ATP + kanamycin A | poor substrate | Pseudomonas aeruginosa | diphosphate + 2''-adenylylkanamycin A | - |
r | |
| ATP + sisomicin | - |
Pseudomonas aeruginosa | diphosphate + 2''-adenylylsisomicin | - |
r | |
| ATP + tobramycin | poor substrate | Pseudomonas aeruginosa | diphosphate + 2''-adenylyltobramycin | - |
r | |
| additional information | amikacin, neomycin, lividomycin and butirosin A and B are no substrates | Pseudomonas aeruginosa | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Pseudomonas aeruginosa |
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