BRENDA - Enzyme Database
show all sequences of 2.7.7.44

Biosynthesis of UDP-glucuronic acid in developing soybean embryos: Possible role of UDP-sugar pyrophosphorylase

Litterer, L.A.; Plaisance, K.L.; Schnurr, J.A.; Storey, K.K.; Jung, H.J.G.; Gronwald, J.W.; Somers, D.A.; Physiol. Plant. 128, 200-211 (2006)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned in Escherichia coli
Glycine max
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
alpha-D-glucuronic acid 1-phosphate
-
Glycine max
0.23
-
alpha-D-glucose 1-phosphate
-
Glycine max
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Glycine max
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71000
-
SDS-PAGE
Glycine max
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
Q09WE7
Soybean (L.) Merrill
-
Purification (Commentary)
Commentary
Organism
His-Link affinity resin
Glycine max
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
UDP-GlcA PPase activity increases 3.9fold during embryo development (25-45 daf), the activity increases in proportion to the increase in seed fresh weight during embryo development
Glycine max
0.0019
-
substrate alpha-D-mannose 1-phosphate
Glycine max
0.0022
-
substrate D-glucose 6-phosphate
Glycine max
0.0034
-
substrate N-acetyl-alpha-D-glucosamine 1-phosphate
Glycine max
0.097
-
substrate alpha-D-glucoronic acid 1-phosphate
Glycine max
0.11
-
substrate alpha-D-glucose 1-phosphate
Glycine max
0.112
-
substrate alpha-D-galactose 1-phosphate
Glycine max
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Glycine max
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
broad pH optimum
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned in Escherichia coli
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
alpha-D-glucuronic acid 1-phosphate
-
Glycine max
0.23
-
alpha-D-glucose 1-phosphate
-
Glycine max
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Glycine max
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71000
-
SDS-PAGE
Glycine max
Purification (Commentary) (protein specific)
Commentary
Organism
His-Link affinity resin
Glycine max
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
UDP-GlcA PPase activity increases 3.9fold during embryo development (25-45 daf), the activity increases in proportion to the increase in seed fresh weight during embryo development
Glycine max
0.0019
-
substrate alpha-D-mannose 1-phosphate
Glycine max
0.0022
-
substrate D-glucose 6-phosphate
Glycine max
0.0034
-
substrate N-acetyl-alpha-D-glucosamine 1-phosphate
Glycine max
0.097
-
substrate alpha-D-glucoronic acid 1-phosphate
Glycine max
0.11
-
substrate alpha-D-glucose 1-phosphate
Glycine max
0.112
-
substrate alpha-D-galactose 1-phosphate
Glycine max
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Glycine max
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
broad pH optimum
Glycine max
Other publictions for EC 2.7.7.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670505
Litterer
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Biosynthesis of UDP-glucuronic ...
Glycine max
Physiol. Plant.
128
200-211
2006
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645284
Amino
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Changes in enzyme activities i ...
Catharanthus roseus
Physiol. Plant.
64
111-117
1985
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645283
Toshinobu
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Purification and properties of ...
Typha latifolia, Typha latifolia Linne
Plant Cell Physiol.
24
1535-1543
1983
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645282
Roberts
The formation of uridine dipho ...
Hordeum vulgare
J. Biol. Chem.
246
4995-5002
1971
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2
2
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5
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1
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1
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1
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3
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2
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5
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1
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1
1
3
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1
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