BRENDA - Enzyme Database
show all sequences of 2.7.7.44

The formation of uridine diphosphate-glucuronic acid in plants. Uridine diphosphate-glucuronic acid pyrophosphorylase from barley seedlings

Roberts, R.M.; J. Biol. Chem. 246, 4995-5002 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory
Hordeum vulgare
Mn2+
53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions
Hordeum vulgare
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
D-glucuronic acid 1-phosphate
pH 8.0, 30°C
Hordeum vulgare
0.5
-
UDP-D-glucuronic acid
pH 8.0, 30°C
Hordeum vulgare
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
36% of the activation with Mg2+
Hordeum vulgare
Co2+
40% of the activation with Mg2+
Hordeum vulgare
Mg2+
best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory
Hordeum vulgare
Mn2+
can substitute for Mg2+ in either the forward or the reverse direction, 53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions
Hordeum vulgare
Zn2+
37% of the activation with Mg2+
Hordeum vulgare
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UTP + D-glucuronic acid 1-phosphate
Hordeum vulgare
predominant route whereby UDPglucuronic acid is termed in young barley seedlings
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hordeum vulgare
-
var. Larker
-
Purification (Commentary)
Commentary
Organism
partial
Hordeum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
-
Hordeum vulgare
Storage Stability
Storage Stability
Organism
-20°C, if dialyzed and stored deep frozen the enzyme rapidly loses activity
Hordeum vulgare
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UTP + D-glucuronic acid 1-phosphate
-
645282
Hordeum vulgare
diphosphate + UDP-D-glucuronic acid
-
645282
Hordeum vulgare
r
UTP + D-glucuronic acid 1-phosphate
at 16% of the activity observed with UTP
645282
Hordeum vulgare
diphosphate + UDP-D-glucuronic acid
-
645282
Hordeum vulgare
r
UTP + D-glucuronic acid 1-phosphate
predominant route whereby UDPglucuronic acid is termed in young barley seedlings
645282
Hordeum vulgare
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Hordeum vulgare
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory
Hordeum vulgare
Mn2+
53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions
Hordeum vulgare
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
D-glucuronic acid 1-phosphate
pH 8.0, 30°C
Hordeum vulgare
0.5
-
UDP-D-glucuronic acid
pH 8.0, 30°C
Hordeum vulgare
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
36% of the activation with Mg2+
Hordeum vulgare
Co2+
40% of the activation with Mg2+
Hordeum vulgare
Mg2+
best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory
Hordeum vulgare
Mn2+
can substitute for Mg2+ in either the forward or the reverse direction, 53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions
Hordeum vulgare
Zn2+
37% of the activation with Mg2+
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UTP + D-glucuronic acid 1-phosphate
Hordeum vulgare
predominant route whereby UDPglucuronic acid is termed in young barley seedlings
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Hordeum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
-
Hordeum vulgare
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, if dialyzed and stored deep frozen the enzyme rapidly loses activity
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UTP + D-glucuronic acid 1-phosphate
-
645282
Hordeum vulgare
diphosphate + UDP-D-glucuronic acid
-
645282
Hordeum vulgare
r
UTP + D-glucuronic acid 1-phosphate
at 16% of the activity observed with UTP
645282
Hordeum vulgare
diphosphate + UDP-D-glucuronic acid
-
645282
Hordeum vulgare
r
UTP + D-glucuronic acid 1-phosphate
predominant route whereby UDPglucuronic acid is termed in young barley seedlings
645282
Hordeum vulgare
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Hordeum vulgare
Other publictions for EC 2.7.7.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670505
Litterer
-
Biosynthesis of UDP-glucuronic ...
Glycine max
Physiol. Plant.
128
200-211
2006
-
-
1
-
-
-
-
2
-
1
1
-
-
1
-
-
1
-
-
-
7
-
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1
-
-
-
1
1
-
-
-
-
-
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-
1
-
-
-
-
-
-
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2
-
1
1
-
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1
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-
7
-
-
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1
-
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1
1
-
-
-
-
-
-
-
-
645284
Amino
-
Changes in enzyme activities i ...
Catharanthus roseus
Physiol. Plant.
64
111-117
1985
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-
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1
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1
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1
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1
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1
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-
-
-
-
-
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-
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-
-
-
-
-
-
645283
Toshinobu
-
Purification and properties of ...
Typha latifolia, Typha latifolia Linne
Plant Cell Physiol.
24
1535-1543
1983
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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1
-
-
-
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-
1
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1
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
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-
645282
Roberts
The formation of uridine dipho ...
Hordeum vulgare
J. Biol. Chem.
246
4995-5002
1971
-
-
-
-
-
-
2
2
-
5
-
1
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
5
-
1
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-