Cloned (Comment) | Organism |
---|---|
gene Cmas, quantitative PCR expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia, phenotype, overview. The Cmas targeting strategy uses a targeting vector with diphtheria toxin cassette (DT) to increase homologous recombination. Cmas-/- mESC accumulate intracellular Neu5Ac. alpha2,3- and alpha2,6-Sialylated N-glycans are absent in Cmas-/- mESCs | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | Mus musculus | - |
diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99KK2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic stem cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Mus musculus | diphosphate + CMP-N-acylneuraminate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CMAS | - |
Mus musculus |
CMP-Sia synthetase | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia show that loss of CMAS activity results in an asialo cell surface accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues, as well as intracellular accumulation of free Sia. These changes do not impact intracellular metabolites or the morphology and transcriptome of pluripotent mESC lines. Moreover, the capacity of Cmas-/- mESCs for undirected differentiation into embryoid bodies, germ layer formation and even the generation of beating cardiomyocytes provides first and conclusive evidence that pluripotency and differentiation of mESC in vitro can proceed in the absence of (poly)sialoglycans. Genetic ablation of CMAS results in complete loss of cellsurface sialylation with concomitant increase in LacNAc structures. Intracellular Neu5Ac accumulation alters neither associated metabolites nor intracellular glycosylation | Mus musculus |
metabolism | the enzyme is involved in the biosynthesis of sialoglycoconjugates in vertebrates, overview | Mus musculus |
physiological function | CMAS activity is essential for maintaining cellular homeostasis of sialic acid biosynthesis | Mus musculus |