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Literature summary for 2.7.7.43 extracted from
- A CMP-N-acetylneuraminic acid synthetase purified from a marine bacterium, Photobacterium leiognathi JT-SHIZ-145 (2011), Biosci. Biotechnol. Biochem., 75, 47-53.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition | Photobacterium leiognathi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.867 | - |
N-acetylneuraminate | pH 9.0, 30°C | Photobacterium leiognathi | |
| 1.57 | - |
CTP | pH 9.0, 30°C | Photobacterium leiognathi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, maximum activity above 5 mM | Photobacterium leiognathi | |
| Mn2+ | 5% of the activity in presence of Mg2+ | Photobacterium leiognathi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55800 | - |
gel filtration | Photobacterium leiognathi |
| 60000 | - |
1 * 60000, SDS-PAGE | Photobacterium leiognathi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photobacterium leiognathi | - |
- |
- |
| Photobacterium leiognathi JT-SHIZ-145 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Photobacterium leiognathi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.165 | - |
30°C, pH 9.0 | Photobacterium leiognathi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + N-acetylneuraminate | - |
Photobacterium leiognathi | diphosphate + CMP-N-acetylneuraminate | - |
? | |
| CTP + N-acetylneuraminate | - |
Photobacterium leiognathi JT-SHIZ-145 | diphosphate + CMP-N-acetylneuraminate | - |
? | |
| CTP + N-glycolylneuraminate | - |
Photobacterium leiognathi | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
| CTP + N-glycolylneuraminate | - |
Photobacterium leiognathi JT-SHIZ-145 | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
| additional information | no substrates: 6'-thioneuraminate, 6-ketodeoxyoctulosonate | Photobacterium leiognathi | ? | - |
? | |
| additional information | no substrates: 6'-thioneuraminate, 6-ketodeoxyoctulosonate | Photobacterium leiognathi JT-SHIZ-145 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Photobacterium leiognathi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Photobacterium leiognathi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Photobacterium leiognathi |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Photobacterium leiognathi | isoelectric focusing | - |
5 |
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Release 2023.1 (January 2023)
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