Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | 1 mM, 20% activation | Mannheimia haemolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Mannheimia haemolytica | |
AMP | 10 mM, 64% inhibition. 20% inhibition by 1 mM | Mannheimia haemolytica | |
ATP | 10 mM, 60% inhibition | Mannheimia haemolytica | |
Ba2+ | 20 mM, less than 30% inhibition | Mannheimia haemolytica | |
Ca2+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
CDP | - |
Mannheimia haemolytica | |
CMP | 50 mM, 58% inhibition | Mannheimia haemolytica | |
Co2+ | 20 mM in presence of 10 mM Mg2+, more than 70% inhibition | Mannheimia haemolytica | |
CTP | - |
Mannheimia haemolytica | |
Cu2+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
cytidine | 60 mM, 37% inhibition | Mannheimia haemolytica | |
Fe3+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
GMP | 20 mM, 64% inhibition | Mannheimia haemolytica | |
GTP | 10 mM, 39% inhibition | Mannheimia haemolytica | |
Hg2+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
Mn2+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
NEM | - |
Mannheimia haemolytica | |
Ni2+ | 20 mM in presence of 10 mM Mg2+, more than 90% inhibition | Mannheimia haemolytica | |
PHMB | 1 mM, complete inhibition | Mannheimia haemolytica | |
Sr2+ | 20 mM, less than 30% inhibition | Mannheimia haemolytica | |
UDP-N-acetylglucosamine | 10 mM, 26% inhibition | Mannheimia haemolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
CTP | pH 9.0, 37°C, reaction with N-glycolylneuraminate | Mannheimia haemolytica | |
1.3 | - |
N-acetylneuraminate methyl ester | pH 9.0, 37°C, reaction with CTP | Mannheimia haemolytica | |
1.6 | - |
N-acetylneuraminate | pH 9.0, 37°C, reaction with TTP | Mannheimia haemolytica | |
1.77 | - |
CTP | pH 9.0, 37°C, reaction with N-acetylneuraminate | Mannheimia haemolytica | |
1.8 | - |
N-acetylneuraminate | pH 9.0, 37°C, reaction with CDP | Mannheimia haemolytica | |
1.82 | - |
N-acetylneuraminate | pH 9.0, 37°C, reaction with CTP | Mannheimia haemolytica | |
1.9 | - |
N-acetylneuraminate | pH 9.0, 37°C, reaction with UTP | Mannheimia haemolytica | |
1.9 | - |
N-acetylneuraminate | pH 9.0, 37°C, reaction with UDP | Mannheimia haemolytica | |
2.1 | - |
CDP | pH 9.0, 37°C, reaction with N-acetylneuraminate | Mannheimia haemolytica | |
2.6 | - |
CTP | pH 9.0, 37°C, reaction with N-acetylneuraminate methyl ester | Mannheimia haemolytica | |
2.7 | - |
UTP | pH 9.0, 37°C, reaction with N-acetylneuraminate | Mannheimia haemolytica | |
3 | - |
N-glycolylneuraminate | pH 9.0, 37°C, reaction with CTP | Mannheimia haemolytica | |
3 | - |
UDP | pH 9.0, 37°C, reaction with N-acetylneuraminate | Mannheimia haemolytica | |
8.1 | - |
TTP | pH 9.0, 37°C, reaction with N-acetylneuraminate | Mannheimia haemolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal ion is strictly required, with 20 mM Ca2+ 13% of the activation compared to activation by Mg2+ | Mannheimia haemolytica | |
Co2+ | divalent metal ion is strictly required, with 20 mM Mn2+ 27% of the activation compared to activation by Mg2+ | Mannheimia haemolytica | |
Mg2+ | divalent metal ion is strictly required, maximal activity at 10 mM | Mannheimia haemolytica | |
Mn2+ | divalent metal ion is strictly required, with 20 mM Mn2+ 28% of the activation compared to activation by Mg2+ | Mannheimia haemolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
3 * 43000, SDS-PAGE | Mannheimia haemolytica |
135000 | - |
gel filtration | Mannheimia haemolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | Mannheimia haemolytica | neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Mannheimia haemolytica A2 | neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase | diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mannheimia haemolytica | - |
A2 | - |
Mannheimia haemolytica A2 | - |
A2 | - |
Purification (Comment) | Organism |
---|---|
- |
Mannheimia haemolytica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + N-acylneuraminate = diphosphate + CMP-N-acylneuraminate | ping pong bi bi mechanism with CTP as the first substrate and CMP-NeuAc as the last product | Mannheimia haemolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + N-acetylneuraminate | - |
Mannheimia haemolytica | phosphate + CMP-N-acetylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Mannheimia haemolytica | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase | Mannheimia haemolytica | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | neuroinvasive and septicaemia-causing pathogens often display a polysialic acid capsule that is involved in invasive behaviour. N-Acetylneuraminate is the basic monomer of polysialic acid. The activated form CMP-N-acylneuraminate is synthesized by N-acylneuraminate cytidylyltransferase | Mannheimia haemolytica A2 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate methyl ester | - |
Mannheimia haemolytica | diphosphate + CMP-N-acylneuraminate methyl ester | - |
? | |
CTP + N-acylneuraminate methyl ester | - |
Mannheimia haemolytica A2 | diphosphate + CMP-N-acylneuraminate methyl ester | - |
? | |
CTP + N-glycolylneuraminate | - |
Mannheimia haemolytica | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
TTP + N-acylneuraminate | - |
Mannheimia haemolytica | diphosphate + TMP-N-acylneuraminate | - |
? | |
TTP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | diphosphate + TMP-N-acylneuraminate | - |
? | |
UDP + N-acetylneuraminate | - |
Mannheimia haemolytica | phosphate + UMP-N-acetylneuraminate | - |
? | |
UTP + N-acylneuraminate | - |
Mannheimia haemolytica | diphosphate + UMP-N-acylneuraminate | - |
? | |
UTP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | diphosphate + UMP-N-acylneuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 43000, SDS-PAGE | Mannheimia haemolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mannheimia haemolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12.5 | activity decreases dramatically below pH 7.0 and above pH 12.5 | Mannheimia haemolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mannheimia haemolytica |