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Literature summary for 2.7.7.43 extracted from

  • Nakata, D.; Munster, A.K.; Gerardy-Schahn, R.; Aoki, N.; Matsuda, T.; Kitajima, K.
    Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates (2001), Glycobiology, 11, 685-692.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oncorhynchus mykiss
expression in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
N-acetylneuraminic acid pH 9.0, 25°C Mus musculus
0.26
-
N-acetylneuraminate pH 9.0, 37°C Mus musculus
0.56
-
3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Mus musculus
0.56
-
2-keto-3-deoxy-D-glycero-D-galacto-nononic acid pH 9.0, 37°C Mus musculus
2.8
-
N-acetylneuraminic acid pH 9.0, 25°C Oncorhynchus mykiss
2.8
-
N-acetylneuraminate pH 9.0, 37°C Oncorhynchus mykiss
3
-
3-deoxy-D-glycero-D-galacto-nononate pH 9.0, 25°C Oncorhynchus mykiss
3
-
2-keto-3-deoxy-D-glycero-D-galacto-nononic acid pH 9.0, 37°C Oncorhynchus mykiss

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
SDS-PAGE, recombinant thioredoxin fusion protein Mus musculus
69000
-
x * 69000, SDS-PAGE Oncorhynchus mykiss
69000
-
SDS-PAGE, recombinant thioredoxin fusion protein Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Mus musculus Q99KK2
-
-
Oncorhynchus mykiss Q90WG6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oncorhynchus mykiss
-
ovary
-
Oncorhynchus mykiss
-
testis
-
Oncorhynchus mykiss
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid enzyme shows high activity towards 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid and towards N-acetylneuraminate Oncorhynchus mykiss diphosphate + CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
-
?
CTP + 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid recombinant enzyme shows 15times lower activity towards 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid than towards N-acetylneuraminate Mus musculus diphosphate + CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
-
?
CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
Oncorhynchus mykiss diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate reaction of EC 2.7.7.92 ?
CTP + 3-deoxy-D-glycero-D-galacto-nononate
-
Mus musculus diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate reaction of EC 2.7.7.92 ?
CTP + N-acetylneuraminic acid
-
Oncorhynchus mykiss diphosphate + CMP-N-acetylneuraminic acid
-
?
CTP + N-acetylneuraminic acid
-
Mus musculus diphosphate + CMP-N-acetylneuraminic acid
-
?
CTP + N-acylneuraminate
-
Oncorhynchus mykiss diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Mus musculus diphosphate + CMP-N-acylneuraminate
-
?

Subunits

Subunits Comment Organism
?
-
Mus musculus
? x * 69000, SDS-PAGE Oncorhynchus mykiss

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
unstable even at Oncorhynchus mykiss