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Literature summary for 2.7.7.42 extracted from

  • Wolf, D.; Ebner, E.; Hinze, H.
    Inactivation, stabilization and some properties of ATP: glutamine synthetase adenylyltransferase from Escherichia coli B (1972), Eur. J. Biochem., 25, 239-244.
    View publication on PubMed
Search for more literature for 2.7.7.42

General Stability

General Stability Organism
bovine serum albumin, above 1 mg/ml, prevents inactivation at 4°C and 25°C and aggregation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information inactivation scheme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-glutamate:ammonia ligase (ADP-forming)] Escherichia coli
-
 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
ATP + [L-glutamate:ammonia ligase (ADP-forming)] Escherichia coli B / ATCC 11303
-
 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 B
-
Escherichia coli B / ATCC 11303
-
 B
-

Storage Stability

Storage Stability Organism
4°C, enzyme solution of 3 mg/ml, loss of 50% activity within 30 days, the activity declines faster with more diluted enzyme solutions Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP)
-
 r
ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli B / ATCC 11303 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)]-(AMP)
-
 r
ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r
ATP + [L-glutamate:ammonia ligase (ADP-forming)]
-
 Escherichia coli B / ATCC 11303 diphosphate + [L-glutamate:ammonia ligase (ADP-forming)] -(AMP)
-
 r

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
 4.2