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Literature summary for 2.7.7.41 extracted from
- Specific inhibition of phosphatidate cytidylyltransferase from Bacillus subtilis membranes by cytidine monophosphate (1983), Biochim. Biophys. Acta, 753, 372-380.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| non-ionic detergent | absolute requirement | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CMP | - |
Bacillus subtilis |  |
| diphosphate | - |
Bacillus subtilis | |
| Hg2+ | 0.1 mM, 90% inhibition | Bacillus subtilis | |
| Mg2+ | 33% inhibition at 25 mM Mg2+ | Bacillus subtilis | |
| PCMB | 0.03 mM, 90% inhibition | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
CTP | pH 7.5, 25°C | Bacillus subtilis | |
| 0.26 | - |
dCTP | pH 7.5, 25°C | Bacillus subtilis | |
| 0.3 | - |
phosphatidic acid | pH 7.5, 25°C, with 0.45 mM CTP | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | localized exclusively in the membrane fraction | Bacillus subtilis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulating effect increases with concentrations up to 75 mM | Bacillus subtilis | |
| Mg2+ | maximal activity with 3-6 mM. 33% inhibition at 25 mM | Bacillus subtilis | |
| Mn2+ | 1 mM, 70% of the maximal activity obtained with Mg2+ | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CTP + phosphatidate = diphosphate + CDP-diacylglycerol | ping-pong mechanism | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 1,2-dipalmitoyl phosphatidic acid | - |
Bacillus subtilis | diphosphate + CDP-dipalmitoylglycerol | - |
? | |
| CTP + phosphatidate | - |
Bacillus subtilis | diphosphate + CDPdiacylglycerol | - |
? | |
| dCTP + phosphatidate | - |
Bacillus subtilis | diphosphate + dCDPdiacylglycerol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
with detergent already inactivated at | Bacillus subtilis |
| 60 | - |
30 min, membranous enzyme, stable up to | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.7 | pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity | Bacillus subtilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
diphosphate | pH 7.5, 25°C | Bacillus subtilis | |
| 5.6 | - |
CMP | pH 7.5, 25°C | Bacillus subtilis | |
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