Literature summary for 2.7.7.4 extracted from

Gay, S.C.; Fribourgh, J.L.; Donohoue, P.D.; Segel, I.H.; Fisher, A.J.
Kinetic properties of ATP sulfurylase and APS kinase from Thiobacillus denitrificans (2009), Arch. Biochem. Biophys., 489, 110-117.

Search for more literature for 2.7.7.4

Cloned(Commentary)

Cloned (Comment)	Organism
His-tag version expressed in Escherichia coli BL21-DE3	Thiobacillus denitrificans ATCC 25259

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0045	-	adenylyl sulfate	ATP synthesis	Thiobacillus denitrificans ATCC 25259
0.029	-	diphosphate	ATP synthesis	Thiobacillus denitrificans ATCC 25259
0.78	-	MgATP2-	adenylyl sulfate synthesis	Thiobacillus denitrificans ATCC 25259
17	-	sulfate	adenylyl sulfate synthesis	Thiobacillus denitrificans ATCC 25259

Organism

Organism	UniProt	Comment	Textmining
Thiobacillus denitrificans ATCC 25259	Q3SEZ6	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography	Thiobacillus denitrificans ATCC 25259

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
31.5	-	adenylyl sulfate synthesis	Thiobacillus denitrificans ATCC 25259
352	-	ATP synthesis	Thiobacillus denitrificans ATCC 25259

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + adenylyl sulfate	-	Thiobacillus denitrificans ATCC 25259	ATP + sulfate	-	r
MgATP2- + sulfate	-	Thiobacillus denitrificans ATCC 25259	Mg-diphosphate + adenylyl sulfate	-	r

Synonyms

Synonyms	Comment	Organism
ATP sulfurylase	-	Thiobacillus denitrificans ATCC 25259

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
23.2	-	sulfate	adenylyl sulfate synthesis	Thiobacillus denitrificans ATCC 25259
260	-	adenylyl sulfate	ATP synthesis	Thiobacillus denitrificans ATCC 25259

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Release 2023.1 (January 2023)